Development and Evaluation of Biodegradable Core-Shell Microfibrous and Nanofibrous Scaffolds for Tissue Engineering Applications.

Autor: Mitropoulou A; Department of Mechanical Engineering and Aeronautics, Laboratory of Biomechanics and Biomedical Engineering, University of Patras, Patras, GR, Greece. athina.mitropoulou@outlook.com., Markatos DN; Department of Mechanical Engineering and Aeronautics, Laboratory of Technology and Strength of Materials, University of Patras, Patras, GR, Greece., Dimopoulos A; Prometheus Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium.; Skeletal Biology and Engineering Research Center, Department of Development and Regeneration, KU Leuven, Leuven, Belgium., Marazioti A; Department of Physiotherapy, Laboratory of Basic Sciences, University of Peloponnese, Sparta, GR, Greece., Mikelis CM; Department of Pharmacy, Laboratory of Molecular Pharmacology University of Patras, Patras, GR, Greece., Mavrilas D; Department of Mechanical Engineering and Aeronautics, Laboratory of Biomechanics and Biomedical Engineering, University of Patras, Patras, GR, Greece.
Jazyk: angličtina
Zdroj: Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2024 Jan 29; Vol. 35 (1), pp. 10. Date of Electronic Publication: 2024 Jan 29.
DOI: 10.1007/s10856-024-06777-z
Abstrakt: Tissue engineering scaffolds as three-dimensional substrates may serve as ideal templates for tissue regeneration by simulating the structure of the extracellular matrix (ECM). Many biodegradable synthetic polymers, either hydrophobic, like Poly-ε-caprolactone (PCL), or hydrophilic, like Poly(Vinyl Alcohol) (PVA), are widely used as candidate bioactive materials for fabricating tissue engineering scaffolds. However, a combination of good cytocompatibility of hydrophilic polymers with good biomechanical performance of hydrophobic polymers could be beneficial for the in vivo performance of the scaffolds. In this study, we aimed to fabricate biodegradable fibrous scaffolds by combining the properties of hydrophobic PCL with those of hydrophilic PVA and evaluate their properties in comparison with pristine PCL scaffolds. Therefore, single-layered PCL scaffolds, sequential tri-layered (PVA/PCL/PVA), and core-shell (PVA as shell and PCL as core) composite scaffolds were developed utilizing the electrospinning technique. The material structural and biomechanical properties of the electrospun scaffolds, before and after their hydrolytic degradation over a seven-month period following storage in phosphate-buffered saline (PBS) at 37 °C, were comprehensively compared. In addition, human embryonic kidney cells (HEK-293) were cultured on the scaffolds to investigate potential cell attachment, infiltration, and proliferation. The results demonstrated the long-term efficacy of core-shell biodegradable fibrous scaffolds in comparison to single-layers PCL and tri-layers PVA/PCL/PVA, not only due to its superior morphological characteristics and mechanical properties, but also due to its ability to promote homogeneous cell distribution and proliferation, without any external chemical or physical stimuli.
(© 2024. The Author(s).)
Databáze: MEDLINE
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