| Autor: |
Madrid Mendoza MF; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil., Almeida Mota J; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil., de Cassia Evangelista de Oliveira F; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Brazil., Cavalcanti BC; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil., Fabio Turco J; Department of Chemistry, Midwestern State University of Guarapuava, Guarapuava, Brazil., Reyes Torres Y; Department of Chemistry, Midwestern State University of Guarapuava, Guarapuava, Brazil., Ferreira PMP; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil., Barros-Nepomuceno FWA; Institute of Health Sciences, University for International Integration of Afro-Brazilian Lusophony, Redenção, Brazil., Rocha DD; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Brazil., Pessoa C; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil., de Moraes Filho MO; Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. |
| Abstrakt: |
Tithonia diversifolia is a perennial bushy plant found in South America with significant ethnopharmacological importance as an antimalarial, antidiabetic, antibacterial, and anticancer agent. The aim of the present study was to determine the cytotoxicity of the ethanolic extract from leaves of T. diversifolia (TdE) on human cancer cell lines (HCT-116, SNB-19, NCIH-460 and MCF-7), as well as the mechanism of action involved in cell death and cellular modulation of oxidative stress. The TdE exhibited significant activity with IC 50 values ranging from 7.12 to 38.41 μg/ml, with HCT-116 being the most sensitive cell line. Subsequent experiments were conducted with HCT-116 cell line. TdE decreased the number of viable cells, followed by induction of apoptotic events, increase in mitochondrial membrane permeabilization, and enhanced G 2 /M phase of the cell cycle. Pro-oxidative effects including elevated acidic vesicular organelle formation, lipid peroxidation, and nitric oxide by-products, as well as reduced levels of intracellular glutathione and reactive oxygen species production were also observed following incubation with TdE, which may lead to DNA damage followed by apoptotic cell death. These results demonstrate the potential of TdE ethanolic leaf extraction for biological activity and enhance the importance of continuing to study natural sources of plants for the development of anticancer agents. |
