Antibiotic-induced gut dysbiosis elicits gut-brain axis relevant multi-omic signatures and behavioral and neuroendocrine changes in a nonhuman primate model.

Autor: Hayer SS; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA., Conrin M; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., French JA; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.; Program in Neuroscience and Behavior, University of Nebraska at Omaha, Omaha, NE, USA., Benson AK; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA., Alvarez S; Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, USA., Cooper K; School of Interdisciplinary Informatics, College of Information Science and Technology, University of Nebraska at Omaha, Omaha, NE, USA., Fischer A; Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, USA., Alsafwani ZW; School of Interdisciplinary Informatics, College of Information Science and Technology, University of Nebraska at Omaha, Omaha, NE, USA., Gasper W; School of Interdisciplinary Informatics, College of Information Science and Technology, University of Nebraska at Omaha, Omaha, NE, USA., Suhr Van Haute MJ; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA., Hassenstab HR; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., Azadmanesh S; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., Briardy M; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., Gerbers S; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., Jabenis A; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., Thompson JL; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA., Clayton JB; Department of Biology, University of Nebraska at Omaha, Omaha, NE, USA.; Callitrichid Research Center, University of Nebraska at Omaha, Omaha, NE, USA.; Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.; Primate Microbiome Project, University of Nebraska-Lincoln, Lincoln, NE, USA.
Jazyk: angličtina
Zdroj: Gut microbes [Gut Microbes] 2024 Jan-Dec; Vol. 16 (1), pp. 2305476. Date of Electronic Publication: 2024 Jan 29.
DOI: 10.1080/19490976.2024.2305476
Abstrakt: Emerging evidence indicates that antibiotic-induced dysbiosis can play an etiological role in the pathogenesis of neuropsychiatric disorders. However, most of this evidence comes from rodent models. The objective of this study was to evaluate if antibiotic-induced gut dysbiosis can elicit changes in gut metabolites and behavior indicative of gut-brain axis disruption in common marmosets ( Callithrix jacchus ) - a nonhuman primate model often used to study sociability and stress. We were able to successfully induce dysbiosis in marmosets using a custom antibiotic cocktail (vancomycin, enrofloxacin and neomycin) administered orally for 28 days. This gut dysbiosis altered gut metabolite profiles, behavior, and stress reactivity. Increase in gut Fusobacterium spp . post-antibiotic administration was a novel dysbiotic response and has not been observed in any rodent or human studies to date. There were significant changes in concentrations of several gut metabolites which are either neurotransmitters (e.g., GABA and serotonin) or have been found to be moderators of gut-brain axis communication in rodent models (e.g., short-chain fatty acids and bile acids). There was an increase in affiliative behavior and sociability in antibiotic-administered marmosets, which might be a coping mechanism in response to gut dysbiosis-induced stress. Increase in urinary cortisol levels after multiple stressors provides more definitive proof that this model of dysbiosis may cause disrupted communication between gut and brain in common marmosets. This study is a first attempt to establish common marmosets as a novel model to study the impact of severe gut dysbiosis on gut-brain axis cross-talk and behavior.
Databáze: MEDLINE