Optical Trapping and Fast Discrimination of Label-Free Bacteriophages at the Single Virion Level.

Autor: Villa N; Institut de Physique, École Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland., Tartari E; Institut de Physique, École Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland., Glicenstein S; Univ. Grenoble Alpes, CEA, Grenoble INP, IRIG, PHELIQS, Grenoble, 38000, France., de Villiers de la Noue H; Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV), Lausanne, 1011, Switzerland., Picard E; Univ. Grenoble Alpes, CEA, Grenoble INP, IRIG, PHELIQS, Grenoble, 38000, France., Marcoux PR; Univ. Grenoble Alpes, CEA, LETI, DTIS, L4IV, Grenoble, 38000, France., Zelsmann M; Université Grenoble Alpes, CNRS, CEA/LETI Minatec, LTM, Grenoble, 38000, France., Resch G; Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV), Lausanne, 1011, Switzerland., Hadji E; Univ. Grenoble Alpes, CEA, Grenoble INP, IRIG, PHELIQS, Grenoble, 38000, France., Houdré R; Institut de Physique, École Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Jul; Vol. 20 (27), pp. e2308814. Date of Electronic Publication: 2024 Jan 28.
DOI: 10.1002/smll.202308814
Abstrakt: There is a recent resurgence of interest in phage therapy (the therapeutic use of bacterial viruses) as an approach to eliminating difficult-to-treat infections. However, existing approaches for therapeutic phage selection and virulence testing are time-consuming, host-dependent, and facing reproducibility issues. Here, this study presents an innovative approach wherein integrated resonant photonic crystal (PhC) cavities in silicon are used as optical nanotweezers for probing and manipulating single bacteria and single virions with low optical power. This study demonstrates that these nanocavities differentiate between a bacterium and a phage without labeling or specific surface bioreceptors. Furthermore, by tailoring the spatial extent of the resonant optical mode in the low-index medium, phage distinction across phenotypically distinct phage families is demonstrated. The work paves the road to the implementation of optical nanotweezers in phage therapy protocols.
(© 2024 The Authors. Small published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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