Dual contrastive learning based image-to-image translation of unstained skin tissue into virtually stained H&E images.

Autor: Asaf MZ; Department of Computer and Software Engineering, National University of Sciences and Technology, Islamabad, Pakistan., Rao B; Center for Dermatology, Rutgers Robert Wood Johnson Medical School, Somerset, NJ, 08873, USA.; Department of Dermatology, Weill Cornell Medicine, New York, NY, 10021, USA., Akram MU; Department of Computer and Software Engineering, National University of Sciences and Technology, Islamabad, Pakistan. usmakram@gmail.com., Khawaja SG; Department of Computer and Software Engineering, National University of Sciences and Technology, Islamabad, Pakistan., Khan S; Center for Dermatology, Rutgers Robert Wood Johnson Medical School, Somerset, NJ, 08873, USA., Truong TM; Center for Dermatology, Rutgers Robert Wood Johnson Medical School, Somerset, NJ, 08873, USA.; Department of Pathology, Immunology and Laboratory Medicine, New Jersey Medical School, 185 South Orange Ave, Newark, NJ, 07103, USA., Sekhon P; EIV Diagnostics, Fresno, CA, USA.; University of California, San Francisco School of Medicine, San Francisco, USA., Khan IJ; Department of Pathology, St. Luke's University Health Network, Bethlehem, PA, 18015, USA., Abbasi MS; Department of Internal Medicine, Greater Baltimore Medical Center, Towson, MD, 21204, USA.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jan 28; Vol. 14 (1), pp. 2335. Date of Electronic Publication: 2024 Jan 28.
DOI: 10.1038/s41598-024-52833-7
Abstrakt: Staining is a crucial step in histopathology that prepares tissue sections for microscopic examination. Hematoxylin and eosin (H&E) staining, also known as basic or routine staining, is used in 80% of histopathology slides worldwide. To enhance the histopathology workflow, recent research has focused on integrating generative artificial intelligence and deep learning models. These models have the potential to improve staining accuracy, reduce staining time, and minimize the use of hazardous chemicals, making histopathology a safer and more efficient field. In this study, we introduce a novel three-stage, dual contrastive learning-based, image-to-image generative (DCLGAN) model for virtually applying an "H&E stain" to unstained skin tissue images. The proposed model utilizes a unique learning setting comprising two pairs of generators and discriminators. By employing contrastive learning, our model maximizes the mutual information between traditional H&E-stained and virtually stained H&E patches. Our dataset consists of pairs of unstained and H&E-stained images, scanned with a brightfield microscope at 20 × magnification, providing a comprehensive set of training and testing images for evaluating the efficacy of our proposed model. Two metrics, Fréchet Inception Distance (FID) and Kernel Inception Distance (KID), were used to quantitatively evaluate virtual stained slides. Our analysis revealed that the average FID score between virtually stained and H&E-stained images (80.47) was considerably lower than that between unstained and virtually stained slides (342.01), and unstained and H&E stained (320.4) indicating a similarity virtual and H&E stains. Similarly, the mean KID score between H&E stained and virtually stained images (0.022) was significantly lower than the mean KID score between unstained and H&E stained (0.28) or unstained and virtually stained (0.31) images. In addition, a group of experienced dermatopathologists evaluated traditional and virtually stained images and demonstrated an average agreement of 78.8% and 90.2% for paired and single virtual stained image evaluations, respectively. Our study demonstrates that the proposed three-stage dual contrastive learning-based image-to-image generative model is effective in generating virtual stained images, as indicated by quantified parameters and grader evaluations. In addition, our findings suggest that GAN models have the potential to replace traditional H&E staining, which can reduce both time and environmental impact. This study highlights the promise of virtual staining as a viable alternative to traditional staining techniques in histopathology.
(© 2024. The Author(s).)
Databáze: MEDLINE
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