| Autor: |
Alotaibi B; Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia., A El-Masry T; Department of Pharmacology and Toxicology, Tanta University, Tanta, Egypt., Elekhnawy E; Department of Pharmaceutical Microbiology, Tanta University, Tanta, Egypt., Mokhtar FA; Department of Pharmacognosy, El Saleheya El Gadida University, Sharkia, Egypt., El-Seadawy HM; Fujairah Research Centre, sakamkam Road, Fujairah, United Arab Emirates., A Negm W; Department of Pharmacognosy, Tanta University, Tanta, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2024 Dec; Vol. 52 (1), pp. 103-113. Date of Electronic Publication: 2024 Jan 27. |
| DOI: |
10.1080/21691401.2024.2306529 |
| Abstrakt: |
The various therapeutic drugs that are currently utilized for the management of cancer, especially breast cancer, are greatly challenged by the augmented resistance that is either acquired or de novo by the cancer cells owing to the long treatment periods. So, this study aimed at elucidating the possible anticancer potential of four compounds 7, 4', 7'', 4'''-tetra- O -methyl amentoflavone, hesperidin, ferulic acid, and chlorogenic acid that are isolated from Cycas thouarsii leaves n -butanol fraction for the first time. The MTT assay evaluated the cytotoxic action of four isolated compounds against MDA-MB-231 breast cancer cells and oral epithelial cells. Interestingly, ferulic acid revealed the lowest IC 50 of 12.52 µg/mL against MDA-MB-231 cells and a high IC 50 of 80.2 µg/mL against oral epithelial cells. Also, using an inverted microscope, the influence of ferulic acid was studied on the MDA-MB-231, which revealed the appearance of apoptosis characteristics like shrinkage of the cells and blebbing of the cell membrane. In addition, the flow cytometric analysis showed that the MDA-MB-231 cells stained with Annexin V/PI had a rise in the count of the cells in the early and late apoptosis stages. Moreover, gel electrophoresis detected DNA fragmentation in the ferulic acid-treated cells. Finally, the effect of the compound was tested at the molecular level by qRT-PCR. An upregulation of the pro-apoptotic genes ( BAX and P53) and a downregulation of the anti-apoptotic gene ( BCL-2 ) were observed. Consequently, our study demonstrated that these isolated compounds, especially ferulic acid, may be vital anticancer agents, particularly for breast cancer, through its induction of apoptosis through the P53-dependent pathway. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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