Autor: |
Thompson AG, Brailsford MA, Beechey RB |
Jazyk: |
angličtina |
Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1987 Feb 27; Vol. 143 (1), pp. 164-9. |
DOI: |
10.1016/0006-291x(87)90645-0 |
Abstrakt: |
Intact maize mesophyll chloroplasts have been isolated in yields of up to 10 mg of chlorophyll per preparation. The chloroplasts were able to reduce 3-phosphoglycerate at a rate of 2.4 mumol of oxygen/min/mg of chlorophyll. This activity was inhibited by preincubating the intact chloroplasts with pyridoxal 5-phosphate. Chloroplast envelopes have been prepared and the protein profile has been obtained on SDS-polyacrylamide gels. The phosphate-translocator from the chloroplast envelope has been identified as a 30kDa polypeptide. |
Databáze: |
MEDLINE |
Externí odkaz: |
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