Lipopolysaccharides of Herbaspirillum species and their relevance for bacterium-host interactions.

Autor: Velichko NS; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia. Electronic address: velichko_n@ibppm.ru., Kokoulin MS; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia., Dmitrenok PS; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia., Grinev VS; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia; Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia., Kuchur PD; Applied Genomics Laboratory, SCAMT Institute, ITMO University, 9 Ulitsa Lomonosova, St. Petersburg 191002, Russia., Komissarov AS; Applied Genomics Laboratory, SCAMT Institute, ITMO University, 9 Ulitsa Lomonosova, St. Petersburg 191002, Russia., Fedonenko YP; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Mar; Vol. 261 (Pt 1), pp. 129516. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1016/j.ijbiomac.2024.129516
Abstrakt: The lipopolysaccharides of Herbaspirillum lusitanum P6-12 T (HlP6-12 T ) and H. frisingense GSF30 T (HfGSF30 T ) was isolated by phenol-water extraction from bacterial cells and was characterized using chemical analysis and SDS-PAGE. It was shown that these bacteria produce LPSs that differ in their physicochemical properties and macromolecular organization. In this paper, the lipid A structure of the HlP6-12 T LPS, was characterized through chemical analyses and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. To prove the effect of the size of micelles on their bioavailability, we examined the activity of both LPSs toward the morphology of wheat seedlings. Analysis of the HlP6-12 T and HfGSF30 T genomes showed no significant differences between the operons that encode proteins involved in the biosynthesis of the lipids A and core oligosaccharides. The difference may be due to the composition of the O-antigen operon. HfGSF30 T has two copies of the rfb operon, with the main one divided into two fragments. In contrast, the HlP6-12 T genome contains only a single rfb-containing operon, and the other O-antigen operons are not comparable at all. The integrity of O-antigen-related genes may also affect LPS variability of. Specifically, we have observed a hairpin structure in the middle of the O-antigen glycosyltransferase gene, which led to the division of the gene into two fragments, resulting in incorrect protein synthesis and potential abnormalities in O-antigen production.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: