Synthesis of phospholipids in human placenta.

Autor: Powell TL; Departments of Pediatrics, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA; Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Theresa.Powell@cuanschutz.edu., Ferchaud-Roucher V; University of Nantes-INRAE UMR 1280 PhAN, CHU Nantes, CRNH Ouest CHU Hotel Dieu, 1 place Alexis Ricordeau, 1er etage aile nord HNB, 44093, Nantes Cedex 1, France. Electronic address: Veronique.Ferchaud-Roucher@univ-nantes.fr., Madi L; Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Lana.Madi@cuanschutz.edu., Uhlson C; Departments of Pediatrics, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Charis.Uhlson@cuanschutz.edu., Zemski-Berry K; Medicine, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Karin.Zemskiberry@cuanschutz.edu., Kramer AC; Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Avery.Kramer@CUAnschutz.edu., Erickson K; Departments of Pediatrics, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Kathryn.Erickson@CUAnschutz.edu., Palmer C; Departments of Pediatrics, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: palmerclr@me.com., Chassen SS; Departments of Pediatrics, University of Colorado Anschutz Medical Campus, 13001 E 17th Pl, Aurora, CO 80045, USA. Electronic address: Stephanie.Chassen@cuanschutz.edu., Castillo-Castrejon M; Department of Pathology, University of Oklahoma Health Sciences Center, 975 NE 10th St., Stanton L Young Biomedical Research Center Room 458, Oklahoma City, OK, 73104, USA. Electronic address: Marisol-Castillocastrejon@ouhsc.edu.
Jazyk: angličtina
Zdroj: Placenta [Placenta] 2024 Mar 06; Vol. 147, pp. 12-20. Date of Electronic Publication: 2024 Jan 21.
DOI: 10.1016/j.placenta.2024.01.007
Abstrakt: Introduction: Placental phospholipid synthesis is critical for the expansion of the placental exchange surface area and for production of signaling molecules. Despite their importance, it is not yet established which enzymes involved in the de novo synthesis and remodeling of placental phospholipids are expressed and active in the human placenta.
Methods: We identified phospholipid synthesis enzymes by immunoblotting in placental homogenates and immunofluorescence in placenta tissue sections. Primary human trophoblast (PHT) cells from term healthy placentas (n = 10) were cultured and exposed to 13 C labeled fatty acids (16:0, 18:1 and 18:2 n-6, 22:6 n-3) for 2 and 24 h. Three phospholipid classes; phosphatidic acid, phosphatidylcholine, and lysophosphatidylcholine containing 13 C fatty acids were quantified by Liquid Chromatography with tandem mass spectrometry (LC/MS-MS).
Results: Acyl transferase and phospholipase enzymes were detected in human placenta homogenate and primarily expressed in the syncytiotrophoblast. Three representative 13 C fatty acids (16:0, 18:1 and 18:2 n-6) were incorporated rapidly into phosphatidic acid in trophoblasts, but 13 C labeled docosahexaenoic acid (DHA; 22:6 n-3) incorporation was not detected. 13 C DHA was incorporated into phosphatidylcholine. Lysophosphatidylcholine containing all four 13 C labeled fatty acids were found in high abundance.
Conclusions: Phospholipid synthesis and remodeling enzymes are present in the syncytiotrophoblast. 13 C labeled fatty acids were rapidly incorporated into cellular phospholipids. 13 C DHA was incorporated into phospholipids through the remodeling pathway rather than by de novo synthesis. These understudied pathways are highly active and critical for structure and function of the placenta.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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