| Autor: |
Díaz-Asencio L; Centro de Estudios Ambientales de Cienfuegos (CEAC), Carretera a Castillo de Jagua Km 1 ½ Ciudad Nuclear, Cienfuegos 59350, Cuba., Chamero-Lago D; Centro de Estudios Ambientales de Cienfuegos (CEAC), Carretera a Castillo de Jagua Km 1 ½ Ciudad Nuclear, Cienfuegos 59350, Cuba., Rojas-Abrahantes GL; Centro de Estudios Ambientales de Cienfuegos (CEAC), Carretera a Castillo de Jagua Km 1 ½ Ciudad Nuclear, Cienfuegos 59350, Cuba., Alonso-Hernández CM; Marine Environment Laboratories, Department of Nuclear Science and Application, International Atomic Energy Agency, 4 Quai Antoine 1er, 98000 Monaco, Monaco., Dechraoui Bottein MY; University of Cote d'Azur, CNRS, ECOSEAS, 28 Avenue Valrose, 06103 Nice, France. |
| Abstrakt: |
Ciguatera, a global issue, lacks adequate capacity for ciguatoxin analysis in most affected countries. The Caribbean region, known for its endemic ciguatera and being home to a majority of the global small island developing states, particularly needs established methods for ciguatoxin detection in seafood and the environment. The radioligand receptor binding assay (r-RBA) is among the in vitro bioassays currently used for ciguatoxin analysis; however, similarly to the other chemical-based or bioassays that have been developed, it faces challenges due to limited standards and interlaboratory comparisons. This work presents a single laboratory validation of an r-RBA developed in a Cuban laboratory while characterizing the performance of the liquid scintillation counter instrument as a key external parameter. The results obtained show the assay is precise, accurate and robust, confirming its potential as a routine screening method for the detection and quantification of ciguatoxins. The new method will aid in identifying high-risk ciguatoxic fish in Cuba and the Caribbean region, supporting monitoring and scientific management of ciguatera and the development of early warning systems to enhance food safety and food security, and promote fair trade fisheries. |
