Effect of Ultraviolet Radiation on the Enzymolytic and Biomechanical Profiles of Abdominal Aortic Adventitia Tissue.

Autor: Arbănaşi EM; Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures (UMFST), 540142 Targu Mures, Romania.; Clinic of Vascular Surgery, Mures County Emergency Hospital, 540136 Targu Mures, Romania.; Department of Vascular Surgery, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania.; Centre for Advanced Medical and Pharmaceutical Research (CCAMF), George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania., Russu E; Clinic of Vascular Surgery, Mures County Emergency Hospital, 540136 Targu Mures, Romania.; Department of Vascular Surgery, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania., Arbănaşi EM; Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures (UMFST), 540142 Targu Mures, Romania., Ciucanu CC; Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures (UMFST), 540142 Targu Mures, Romania.; Clinic of Vascular Surgery, Mures County Emergency Hospital, 540136 Targu Mures, Romania., Mureșan AV; Clinic of Vascular Surgery, Mures County Emergency Hospital, 540136 Targu Mures, Romania.; Department of Vascular Surgery, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania., Suzuki S; Centre for Advanced Medical and Pharmaceutical Research (CCAMF), George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania.; Queensland Eye Institute, South Brisbane, QLD 4101, Australia., Chirilă TV; Centre for Advanced Medical and Pharmaceutical Research (CCAMF), George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania.; Queensland Eye Institute, South Brisbane, QLD 4101, Australia.; Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania.; School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4001, Australia.; Australian Institute of Bioengineering and Nanotechnology (AIBN), University of Queensland, St. Lucia, QLD 4072, Australia.
Jazyk: angličtina
Zdroj: Journal of clinical medicine [J Clin Med] 2024 Jan 22; Vol. 13 (2). Date of Electronic Publication: 2024 Jan 22.
DOI: 10.3390/jcm13020633
Abstrakt: Background: The abdominal aortic aneurysm (AAA) is defined as an increase in aortic diameter by more than 50% and is associated with a high risk of rupture and mortality without treatment. The aim of this study is to analyze the role of aortic adventitial collagen photocrosslinking by UV-A irradiation on the biomechanical profile of the aortic wall.
Methods: This experimental study is structured in two parts: the first part includes in vitro uniaxial biomechanical evaluation of porcine adventitial tissue subjected to either short-term elastolysis or long-term collagenolysis in an attempt to duplicate two extreme situations as putative stages of aneurysmal degeneration. In the second part, we included biaxial biomechanical evaluation of in vitro human abdominal aortic adventitia and human AAA adventitia specimens. Biomechanical profiles were examined for porcine and human aortic tissue before and after irradiation with UV-A light (365 nm wavelength).
Results: On the porcine aortic sample, the enhancing effect of irradiation was evident both on the tissue subjected to elastolysis, which had a high collagen-to-elastin ratio, and on the tissue subjected to prolonged collagenolysis despite being considerably depleted in collagen. Further, the effect of irradiation was conclusively demonstrated in the human adventitia samples, where significant post-irradiation increases in Cauchy stress (longitudinal axis: p = 0.001, circumferential axis: p = 0.004) and Young's modulus (longitudinal axis: p = 0.03, circumferential axis: p = 0.004) were recorded. Moreover, we have a stronger increase in the strengthening of the AAA adventitia samples following the exposure to UV-A irradiation ( p = 0.007) and a statistically significant but not very important increase ( p = 0.021) regarding the stiffness in the circumferential axis.
Conclusions: The favorable effect of UV irradiation on the strength and stiffness of degraded aortic adventitia in experimental situations mimicking early and later stages of aneurysmal degeneration is essential for the development and potential success of procedures to prevent aneurysmal ruptures. The experiments on human normal and aneurysmal adventitial tissue confirmed the validity and potential success of a procedure based on exposure to UV-A radiation.
Databáze: MEDLINE
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