Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: A snapshot study.

Autor: Morsli M; Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Nîmes, France., Salipante F; Department of Biostatistics, Epidemiology, Public Health, and Innovation in Methodology (BESPIM), CHU Nîmes, Univ Montpellier, Nîmes, France., Gelis A; Centre Mutualiste Neurologique Propara, Montpellier, France., Magnan C; VBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Nîmes, France., Guigon G; bioMérieux SA, Marcy-l'Etoile, France., Lavigne JP; VBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Nîmes, France., Sotto A; VBIC, INSERM U1047, Department of Infectious and Tropical Diseases, CHU Nîmes, Univ Montpellier, Nîmes, France., Dunyach-Remy C; VBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Nîmes, France.
Jazyk: angličtina
Zdroj: International wound journal [Int Wound J] 2024 Jan; Vol. 21 (1), pp. e14626.
DOI: 10.1111/iwj.14626
Abstrakt: Current microbiome investigations of patients with pressure ulcers (PU) are mainly based on wound swabs and/or biopsy sequencing, leaving the colonization scenario unclear. Urinary microbiota has been never studied. As a part of the prospective ESCAFLOR study, we studied urinary microbiota of spinal cord injury (SCI) patients with PU without any urinary tract infection at the inclusion, collected at two times (at admission [D0] and after 28 days [D28]) during the patient's care, investigated by 16S rDNA metagenomics next generation sequencing. Subgroup analyses were carried out between patients with wounds showing improved evolution versus stagnated/worsened wounds at D28. Analysis was done using EPISEQ® 16S and R software. Among the 12 studied patients, the urinary microbiota of patients with improved wound evolution at D28 (n = 6) presented a significant decrease of microbial diversity. This modification was associated with the presence of Proteobacteria phylum and an increase of Escherichia-Shigella (p = 0.005), as well as the presence of probiotic anaerobic bacteria Lactobacillus and Bifidobacterium. In contrast, Proteus abundance was significantly increased in urine of patients with stagnated/worsened wound evolution (n = 6) (p = 0.003). This study proposes urinary microbiota as a complementary factor indirectly associated with the wound evolution and patient cure. It opens new perspectives for further investigations based on multiple body microbiome comparison to describe the complete scenario of the transmission dynamics of wound-colonizing microorganisms.
(© 2024 The Authors. International Wound Journal published by Medicalhelplines.com Inc and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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