Influence of pre-B cell receptor deficiency on the immunoglobulin repertoires in peripheral blood B cells before and after immunization.

Autor: Shimizu T; Department of Immunology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan. Electronic address: tshimizu@kochi-u.ac.jp., Sun L; Department of Immunology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan., Ohnishi K; Department of Immunology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan.
Jazyk: angličtina
Zdroj: Molecular immunology [Mol Immunol] 2024 Feb; Vol. 166, pp. 87-100. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1016/j.molimm.2024.01.001
Abstrakt: During B cell development, pre-B cell receptor (pre-BCR), comprising the immunoglobulin heavy chain (HC) and surrogate light chain (SLC), plays a crucial role. The expression of pre-BCR serves as a certification of HC quality, confirming its ability to associate with the SLC and light chain (LC). In mice lacking SLC, the absence of this quality control mechanism leads to a distorted repertoire of HCs in the spleen and bone marrow. In this study, we conducted a comparative analysis of the immunoglobulin gene repertoire in peripheral blood cells of both wild-type mice and pre-BCR-deficient mice. Our findings reveal differences not only in the μ HC repertoire but also in the α HC and κ LC repertoires of the pre-BCR-deficient mice. These results suggest that the pre-BCR-mediated quality check of HC influences the selection of class-switched HC and LC repertoires. To further explore the impact of pre-BCR deficiency, we immunized these mice with thymus-dependent antigens and compared the antigen-responding repertoires. Our observations indicate that the affinity maturation pathways remain consistent between wild-type mice and pre-BCR-deficient mice, albeit with variations in the degree of maturation.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflicts of interest with the contents of this article.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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