IFNL1 rs30461 polymorphism as a risk factor for COVID-19 severity: A cross-sectional study.

Autor: Hammad MO; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: maha_osman@mans.edu.eg., Alseoudy MM; Department of Anesthesia and Surgical ICU, Mansoura University Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Borg AM; Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., El-Mesery A; Tropical Medicine Department, Mansoura University Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Elgamal M; Chest Medicine Department, Mansoura University Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Abdelghany DA; Chest Medicine Department, Mansoura University Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Elzeiny D; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Jazyk: angličtina
Zdroj: Cytokine [Cytokine] 2024 Apr; Vol. 176, pp. 156500. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1016/j.cyto.2024.156500
Abstrakt: Introduction: The molecular basis of the progression of some COVID-19 patients to worse outcomes is not entirely known. Interferons-lambda-1/interleukin-29 (IFN-λ1/IL-29) is a member of the type III IFNs with a strong antiviral activity. Given the scant data on the potential role of IFN-λ1/IL-29 in COVID-19, we investigated the association of IFN-λ1/IL-29 serum level and the IFNL1 single-nucleotide polymorphism (SNP) (rs30461) with severe course of COVID-19.
Material and Methods: This cross-sectional study included 400 COVID-19 patients, in which 262 mild COVID-19 patients and 138 severe COVID-19 patients were recruited and compared. The IFN-λ1/IL-29 serum levels were assessed in both the mild and severe COVID-19 groups. All participants were genotyped for the IFNL1 SNP (rs30461) by allelic discrimination RT-PCR using specific Taqman probes and primers. The associations between IFNL1 variants and risk of severe COVID-19 were examined via the logistic regression analysis.
Results: The serum IFN-λ1/IL-29 levels showed no statistically significant difference between mild and severe COVID-19 patients (P = 0.993). The genotype and allele frequencies of IFNL1 SNP (rs30461) were significantly different between the mild and severe groups, in which the minor G allele carried a highly significant risk of severe COVID-19 compared with the wild A allele [OR (95 %CI): 2.1 (1.5-2.9), P ≤ 0.001]. In multivariate analysis, the A/G and G/G genotypes of IFNL1 SNP (rs30461) were independent predictors of COVID-19 severity (P < 0.05).
Conclusion: The study concluded that the IFNL1 SNP (rs30461) may constitute an independent risk factor for COVID-19 severity.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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