The forkhead transcription factor Foxj1 controls vertebrate olfactory cilia biogenesis and sensory neuron differentiation.

Autor: Rayamajhi D; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore., Ege M; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway., Ukhanov K; Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida, United States of America., Ringers C; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway., Zhang Y; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore., Jung I; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.; Department of Biomedical Sciences, Korea University, Ansan, South Korea., D'Gama PP; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway., Li SS; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore., Cosacak MI; German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany., Kizil C; Department of Neurology and The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Irving Medical Center, New York, New York, United States of America., Park HC; Department of Biomedical Sciences, Korea University, Ansan, South Korea., Yaksi E; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway.; Koç University Research Center for Translational Medicine, Koç University School of Medicine, Istanbul, Turkey., Martens JR; Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida, United States of America., Brody SL; Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America., Jurisch-Yaksi N; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.; Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway., Roy S; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore.; Department of Biological Sciences, National University of Singapore, Singapore.; Department of Paediatrics, National University of Singapore, Singapore.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2024 Jan 25; Vol. 22 (1), pp. e3002468. Date of Electronic Publication: 2024 Jan 25 (Print Publication: 2024).
DOI: 10.1371/journal.pbio.3002468
Abstrakt: In vertebrates, olfactory receptors localize on multiple cilia elaborated on dendritic knobs of olfactory sensory neurons (OSNs). Although olfactory cilia dysfunction can cause anosmia, how their differentiation is programmed at the transcriptional level has remained largely unexplored. We discovered in zebrafish and mice that Foxj1, a forkhead domain-containing transcription factor traditionally linked with motile cilia biogenesis, is expressed in OSNs and required for olfactory epithelium (OE) formation. In keeping with the immotile nature of olfactory cilia, we observed that ciliary motility genes are repressed in zebrafish, mouse, and human OSNs. Strikingly, we also found that besides ciliogenesis, Foxj1 controls the differentiation of the OSNs themselves by regulating their cell type-specific gene expression, such as that of olfactory marker protein (omp) involved in odor-evoked signal transduction. In line with this, response to bile acids, odors detected by OMP-positive OSNs, was significantly diminished in foxj1 mutant zebrafish. Taken together, our findings establish how the canonical Foxj1-mediated motile ciliogenic transcriptional program has been repurposed for the biogenesis of immotile olfactory cilia, as well as for the development of the OSNs.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Rayamajhi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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