Encyclopedia of Family A DNA Polymerases Localized in Organelles: Evolutionary Contribution of Bacteria Including the Proto-Mitochondrion.

Autor:	Harada R; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan., Hirakawa Y; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan., Yabuki A; Deep-Sea Biodiversity Research Group, Research Institute for Global Change (RIGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan., Kim E; Division of EcoScience, Ewha Womans University, Seoul, South Korea.; Division of Invertebrate Zoology, American Museum of Natural History, New York, NY, USA., Yazaki E; Research Center for Advanced Analysis, National Agriculture and Food Research Organization, Tsukuba, Japan.; Interdisciplinary Theoretical and Mathematical Sciences program (iTHEMS), RIKEN, Wako, Saitama, Japan., Kamikawa R; Graduate School of Agriculture, Kyoto University, Kyoto, Japan., Nakano K; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan., Eliáš M; Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic., Inagaki Y; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.; Center for Computational Sciences, University of Tsukuba, Tsukuba, Japan.
Jazyk:	angličtina
Zdroj:	Molecular biology and evolution [Mol Biol Evol] 2024 Feb 01; Vol. 41 (2).
DOI:	10.1093/molbev/msae014
Abstrakt:	DNA polymerases synthesize DNA from deoxyribonucleotides in a semiconservative manner and serve as the core of DNA replication and repair machinery. In eukaryotic cells, there are 2 genome-containing organelles, mitochondria, and plastids, which were derived from an alphaproteobacterium and a cyanobacterium, respectively. Except for rare cases of genome-lacking mitochondria and plastids, both organelles must be served by nucleus-encoded DNA polymerases that localize and work in them to maintain their genomes. The evolution of organellar DNA polymerases has yet to be fully understood because of 2 unsettled issues. First, the diversity of organellar DNA polymerases has not been elucidated in the full spectrum of eukaryotes. Second, it is unclear when the DNA polymerases that were used originally in the endosymbiotic bacteria giving rise to mitochondria and plastids were discarded, as the organellar DNA polymerases known to date show no phylogenetic affinity to those of the extant alphaproteobacteria or cyanobacteria. In this study, we identified from diverse eukaryotes 134 family A DNA polymerase sequences, which were classified into 10 novel types, and explored their evolutionary origins. The subcellular localizations of selected DNA polymerases were further examined experimentally. The results presented here suggest that the diversity of organellar DNA polymerases has been shaped by multiple transfers of the PolI gene from phylogenetically broad bacteria, and their occurrence in eukaryotes was additionally impacted by secondary plastid endosymbioses. Finally, we propose that the last eukaryotic common ancestor may have possessed 2 mitochondrial DNA polymerases, POP, and a candidate of the direct descendant of the proto-mitochondrial DNA polymerase I, rdxPolA, identified in this study. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.)
Databáze:	MEDLINE
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