| Autor: |
Javaid N; Department of Life Sciences, School of Science and Engineering, Lahore University of Management Science, Lahore, Pakistan.; Parasites and Microbes, Wellcome Sanger Institute, Hinxton, UK., Lo SW; Parasites and Microbes, Wellcome Sanger Institute, Hinxton, UK.; Milner Centre for Evolution, Department of Life Science, University of Bath, Bath, UK., Nisar MI; Departments of Pathology, Pediatrics, and Medicine, Aga Khan University, Karachi, Pakistan., Basharat A; Department of Life Sciences, School of Science and Engineering, Lahore University of Management Science, Lahore, Pakistan., Jaleel H; Department of Life Sciences, School of Science and Engineering, Lahore University of Management Science, Lahore, Pakistan., Rasool K; Department of Microbiology, Chughtai Lab/Chughtai Institute of Pathology, Lahore, Pakistan., Sultana Q; Department of Microbiology, Chughtai Lab/Chughtai Institute of Pathology, Lahore, Pakistan., Kabir F; Infectious Diseases Research Laboratory (IDRL), Dept. of Paediatrics & Child Health, Aga Khan University, Karachi, Pakistan., Hotwani A; Departments of Pathology, Pediatrics, and Medicine, Aga Khan University, Karachi, Pakistan., Breiman RF; Department of Global Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA., Bentley SD; Parasites and Microbes, Wellcome Sanger Institute, Hinxton, UK., Shakoor S; Departments of Pathology, Pediatrics, and Medicine, Aga Khan University, Karachi, Pakistan., Mirza S; Department of Life Sciences, School of Science and Engineering, Lahore University of Management Science, Lahore, Pakistan. |
| Abstrakt: |
Pakistan is amongst the four countries with the highest number of pneumococcal deaths. While the PCV10 vaccine was introduced in Pakistan in October 2012, data regarding the impact of the vaccine on the population dynamics of Streptococcus pneumoniae in Pakistan remain obscure. Using whole genome sequencing of 190 isolates (nasopharyngeal carriage=75, disease=113, unknown sites=2) collected between 2002 and 2020, this study presents characteristics of pneumococcal strains in Pakistan in the pre- and post-vaccine era. The isolates were characterized on the basis of serotype distribution, genetic lineages (or Global Pneumococcal Sequence Cluster, GPSC) and antibiotic resistance. A high level of diversity in serotype and genetic lineages of pneumococci was observed in Pakistan. Among 190 isolates, we identified 54 serotypes, 67 GPSCs and 116 sequence types (STs) including 23 new STs. The most prevalent GPSCs and their associated serotypes in nasopharyngeal carriage were GPSC54 (expressing serotype 9V), GPSC5 (15A and 7B, and serogroup 24), GPSC25 (15B/15C), GPSC67 (18C) and GPSC376 (6A and 6D). Similarly, among 113 disease-causing isolates, the most prevalent GPSC/serotype combinations were GPSC2 (serotype 1), GPSC10 (serotypes 14, 10A, 19A and 19F), GPSC43 (serotypes 13, 11A, 23B, 35A and 9V), GPSC67 (serotypes 18A and 18C) and GPSC642 (serotype 11A). Of the 190 isolates, the highest levels of resistance were observed against penicillin (58.9 %, n =122), erythromycin (29.5 %, n =56), clindamycin (13.2 %, n =25), co-trimoxazole (94.2 %, n =179) and tetracycline/doxycycline (53.2 %, n =101). A higher proportion of disease-causing isolates were multidrug resistant as compared to carriage isolates (54 % vs 25 %). Our data suggest limited coverage of PCV10 in nasopharyngeal (21.6 %, 16/74) as well as disease-causing (38.1 %, 16/42) isolates among children ≤5 years old; however, higher valent vaccine PCV13 would increase the coverage rates to 33.8 % in nasopharyngeal and 54.8 % in disease-causing isolates, whereas PCV24/25 would offer the highest coverage rates. Owing to the diversity of serotypes observed during the post-vaccine period, the suggested inclusion of serotype in future vaccine formulations will require investigations with larger data sets with an extended temporal window. This article contains data hosted by Microreact. |
