Hydroxymethylnitrofurazone lymphatic uptake with nanostructured lipid carrier after oral administration in rats.

Autor: Souza A; Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, 05508-000, Brazil., Scarim CB; Department of Drugs & Medicines, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, 14800-901, Brazil., Cotrim PC; Seroepidemiology, Cellular & Molecular Immunology Laboratory, Institute of Tropical Medicine, University of São Paulo, Dr. Enéas de Carvalho Aguiar 470, Jardim América, São Paulo, SP, 05403-000, Brazil., Junior FB; Laboratory of Toxicology & Essentiality of Metals, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-903, Brazil., Rocha BA; Laboratory of Toxicology & Essentiality of Metals, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-903, Brazil., Calixto LA; Federal University of São Paulo, Department of Pharmaceutical Sciences, Institute of Environmental, Chemical & Pharmaceutical Sciences, Diadema - SP, 09913-030, Brazil., Correia CJ; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, 01246-903, Brazil., de Barros Araújo GL; Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, 05508-000, Brazil., Löbenberg R; University of Alberta, Faculty of Pharmacy & Pharmaceutical Sciences, Edmonton, AB, T6G 2T9, Canada., Bou-Chacra NA; Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, 05508-000, Brazil., Breithaupt-Faloppa AC; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, 01246-903, Brazil.
Jazyk: angličtina
Zdroj: Nanomedicine (London, England) [Nanomedicine (Lond)] 2024 Feb; Vol. 19 (4), pp. 293-301. Date of Electronic Publication: 2024 Jan 25.
DOI: 10.2217/nnm-2023-0263
Abstrakt: Background: Leishmaniasis, caused by the protozoan Leishmania sp., infects phagocyte cells present in lymphatic organs. This study demonstrates the influence of nanostructured lipid carrier-loaded hydroxymethylnitrofurazone (NLC-NFOH) on lymphatic uptake using a chylomicron-blocking flow model in rats. Method: Lymphatic uptake of NFOH was assessed 1 h after oral administration of dimethyl sulfoxide with NFOH or NLC-NFOH with and without cycloheximide pretreatment. Result: Dimethyl sulfoxide with NFOH and NLC-NFOH showed NFOH serum concentrations of 0.0316 and 0.0291 μg/ml, respectively. After chylomicron blocking, NFOH was not detected. Conclusion: Despite log P below 5, NFOH was successfully taken up by the lymphatic system. Long-chain fatty acids and particle size might be main factors in these findings. NLC-NFOH is a promising and convenient platform for treating leishmaniasis via oral administration.
Databáze: MEDLINE