Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.

Autor:	Morozumi Y; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Mahayot F; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Nakase Y; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Soong JX; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Yamawaki S; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Sofyantoro F; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.; Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta 55281, Indonesia., Imabata Y; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Oda AH; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan., Tamura M; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan., Kofuji S; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Akikusa Y; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Shibatani A; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan., Ohta K; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan., Shiozaki K; Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.; Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Dec 26; Vol. 27 (1), pp. 108777. Date of Electronic Publication: 2023 Dec 26 (Print Publication: 2024).
DOI:	10.1016/j.isci.2023.108777
Abstrakt:	Cells cease to proliferate above their growth-permissible temperatures, a ubiquitous phenomenon generally attributed to heat damage to cellular macromolecules. We here report that, in the presence of rapamycin, a potent inhibitor of Target of Rapamycin Complex 1 (TORC1), the fission yeast Schizosaccharomyces pombe can proliferate at high temperatures that usually arrest its growth. Consistently, mutations to the TORC1 subunit RAPTOR/Mip1 and the TORC1 substrate Sck1 significantly improve cellular heat resistance, suggesting that TORC1 restricts fission yeast growth at high temperatures. Aiming for a more comprehensive understanding of the negative regulation of high-temperature growth, we conducted genome-wide screens, which identified additional factors that suppress cell proliferation at high temperatures. Among them is Mks1, which is phosphorylated in a TORC1-dependent manner, forms a complex with the 14-3-3 protein Rad24, and suppresses the high-temperature growth independently of Sck1. Our study has uncovered unexpected mechanisms of growth restraint even below the temperatures deleterious to cell physiology. Competing Interests: The authors declare no competing interests. (© 2023 The Author(s).)
Databáze:	MEDLINE
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