Lactobacillus rhamnosus dampens cytokine and chemokine secretion from primary human nasal epithelial cells infected with rhinovirus.

Autor:	Yarlagadda T; Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane 4000, Australia., Zhu Y; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia 4072, Australia., Snape N; University of Queensland Frazer Institute, Woolloongabba 4102, Australia., Carey A; Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane 4000, Australia., Bryan E; Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane 4000, Australia.; Faculty of Medicine, University of Queensland Centre for Clinical Research, Herston 4006, Australia., Maresco-Pennisi D; Faculty of Medicine, University of Queensland Centre for Clinical Research, Herston 4006, Australia., Coleman A; Faculty of Medicine, University of Queensland Centre for Clinical Research, Herston 4006, Australia., Cervin A; Faculty of Medicine, University of Queensland Centre for Clinical Research, Herston 4006, Australia., Spann K; Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane 4000, Australia.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2024 Feb 01; Vol. 135 (2).
DOI:	10.1093/jambio/lxae018
Abstrakt:	Aims: To investigate the effect of Lactobacillus rhamnosus on viral replication and cellular response to human rhinovirus (HRV) infection, including the secretion of antiviral and inflammatory mediators from well-differentiated nasal epithelial cells (WD-NECs). Methods and Results: The WD-NECs from healthy adult donors (N = 6) were cultured in vitro, exposed to different strains of L. rhamnosus (D3189, D3160, or LB21), and infected with HRV (RV-A16) after 24 h. Survival and adherence capacity of L. rhamnosus in a NEC environment were confirmed using CFSE-labelled isolates, immunofluorescent staining, and confocal microscopy. Shed virus and viral replication were quantified using TCID50 assays and RT-qPCR, respectively. Cytotoxicity was measured by lactate dehydrogenase (LDH) activity. Pro-inflammatory mediators were measured by multiplex immunoassay, and interferon (IFN)-λ1/3 was measured using a standard ELISA kit. Lactobacillus rhamnosus was able to adhere to and colonize WD-NECs prior to the RV-A16 infection. Lactobacillus rhamnosus did not affect shed RV-A16, viral replication, RV-A16-induced IFN-λ1/3 production, or LDH release. Pre-exposure to L. rhamnosus, particularly D3189, reduced the secretion of RV-A16-induced pro-inflammatory mediators by WD-NECs. Conclusions: These findings demonstrate that L. rhamnosus differentially modulates RV-A16-induced innate inflammatory immune responses in primary NECs from healthy adults. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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