Development of porcine embryos cultured in media irradiated with ultraviolet-C.

Autor: Torigoe N; Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan., Nagahara M; Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan., Nguyen ST; Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan., Lin Q; Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan., Takebayashi K; Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan., Liu B; Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan., Aihara M; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan., Taniguchi M; Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan., Otoi T; Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 Jan; Vol. 59 (1), pp. e14520.
DOI: 10.1111/rda.14520
Abstrakt: Sterilization of the culture medium using ultraviolet (UV)-C reduces the potential adverse effects of microorganisms and allows for long-term use. In the present study, we investigated the effects of a medium directly irradiated with UV-C prior to in vitro culture on the development and quality of porcine in vitro-fertilized embryos and the free amino acid composition of the culture media. The culture media (porcine zygote medium [PZM-5] and porcine blastocyst medium [PBM]) were irradiated with UV-C at 228 and 260 nm for 1 and 3 days, respectively. Next, the culture media were irradiated with UV-C at 228 nm for 3, 7, or 14 days. After in vitro fertilization, the embryos were cultured in the UV-C-irradiated media for 7 days. Free amino acid levels in culture media irradiated with 228 and 260 nm UV-C for 3 days were analysed. The blastocyst formation rate of embryos cultured in media irradiated with 260 nm UV-C for 3 days was significantly lower than that of embryos cultured in non-irradiated control media. However, 228 nm UV-C irradiation for up to 14 days did not affect blastocyst formation rates and quality in the resulting blastocysts. Moreover, 260 nm UV-C irradiation significantly increased the taurine concentration in both culture media and decreased methionine concentration in the PBM. In conclusion, UV-C irradiation at 228 nm before in vitro culture had no detrimental effects on embryonic development. However, 260 nm UV-C irradiation decreased embryo development and altered the composition of free amino acids in the medium.
(© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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