Effect of Five Different Antioxidants on the Effectiveness of Goat Semen Cryopreservation.

Autor: Yi X; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China., Qiu Y; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China., Tang X; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China., Lei Y; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China., Pan Y; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China., Raza SHA; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.; Guangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou, 510642, China.; Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan, 512005, China., Althobaiti NA; Biology Department, College of Science and Humanities, Shaqra University, Al Quwaiiyah, 19257, Al Quwaiiyah, Saudi Arabia., Albalawi AE; Faculty of Science, Department of Biology, University of Tabuk, 47913, Tabuk, Saudi Arabia., Al Abdulmonem W; Department of Pathology, College of Medicine, Qassim University, P.O. Box 6655, Buraidah, 51452, Kingdom of Saudi Arabia., Makhlof RTM; Department of Parasitology, Faculty of Medicine, Umm Al Qura University, P.O. Box 715, 21955, Makkah, Saudi Arabia., Alsaad MA; College of Medicine, Umm AL Qura University, 21955, Makkah, Saudi Arabia., Zhang Y; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China., Sun X; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. sunxiuzhu@nwafu.edu.cn.; College of Grassland Agriculture, Northwest A&F University, Yangling, China. sunxiuzhu@nwafu.edu.cn.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 Jul; Vol. 31 (7), pp. 1958-1972. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1007/s43032-024-01452-8
Abstrakt: The effective combination of semen cryopreservation and artificial insemination has a positive effect on the conservation of germplasm resources, production and breeding, etc. However, during the process of semen cryopreservation, the sperm cells are very susceptible to different degrees of physical, chemical, and oxidative stress damage. Oxidative damage is the most important factor that reduces semen quality, which is affected by factors such as dilution equilibrium, change of osmotic pressure, cold shock, and enzyme action during the freezing-thawing process, which results in the aggregation of a large amount of reactive oxygen species (ROS) in sperm cells and affects the quality of semen after thawing. Therefore, the method of adding antioxidants to semen cryoprotective diluent is usually used to improve the effect of semen cryopreservation. The aim of this experiment was to investigate the effects of adding five antioxidants (GLP, Mito Q, NAC, SLS, and SDS) to semen cryoprotection diluent on the cryopreservation effect of semen from Saanen dairy goats. The optimal preservation concentrations were screened by detecting sperm viability, plasma membrane integrity, antioxidant capacity, and acrosomal enzyme activities after thawing, and the experimental results were as follows: the optimal concentrations of GLP, Mito Q, NAC, SLS, and SDS added to semen cryopreservation diluent at different concentrations were 0.8 mg/mL, 150 nmol/L, 0.6 mg/mL, 0.15 mg/ mL, 0.6 mg/mL, and 0.15 mg/mL. The optimal concentrations of the five antioxidants were added to the diluent and analyzed after 1 week of cryopreservation, and it was found that sperm viability, plasma membrane integrity, and mitochondrial activity were significantly enhanced after thawing compared with the control group (P < 0.05), and their antioxidant capacity was significantly enhanced (P < 0.05). Therefore, the addition of the above five antioxidants to goat sperm cryodilution solution had a better enhancement of sperm cryopreservation. This study provides a useful reference for exploring the improvement of goat semen cryoprotection effect.
(© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
Databáze: MEDLINE
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