Aquaporins (AQPs) as a marker in the physiology of inflammation and its interaction studies with garcinol.
Autor: | Kalita A; Department of Zoology, Animal Physiology and Biochemistry Laboratory, Gauhati University, Guwahati, Assam, 781014, India., Das M; Department of Zoology, Animal Physiology and Biochemistry Laboratory, Gauhati University, Guwahati, Assam, 781014, India. manasdasne@gmail.com. |
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Jazyk: | angličtina |
Zdroj: | Inflammopharmacology [Inflammopharmacology] 2024 Apr; Vol. 32 (2), pp. 1575-1592. Date of Electronic Publication: 2024 Jan 24. |
DOI: | 10.1007/s10787-023-01412-9 |
Abstrakt: | Aquaporins like AQP1, AQP3, and AQP4 are known to be involved in the pathophysiology of inflammation based on earlier reports. This study aimed to evaluate the involvement of Aquaporins as a potential target of inflammation. The study also investigates the efficacy of methanolic extract of Garcinia (GME) and its potent phytocompound (garcinol) against the Aquaporins involved in inflammation. siRNA silencing of AQP3 was carried out in RAW264.7 cells followed by LPS stimulation (1 µg/ml) and assessment of important markers of inflammation including NO, PGE2, TNF-α, IL-6, IL-1β, CCL20, iNOS and COX-2. To assess the anti-inflammatory potential of Garcinia extract and garcinol, cells were stimulated with 1 µg/ml LPS in the absence and presence of increasing concentrations of GME and garcinol. During the experimental period, extract concentrations (115 µg/ml and 230 µg/ml for RAW264.7; 118 µg/ml and 236 µg/ml for THP-1) and garcinol concentrations (6 µM and 12 µM for RAW264.7; 3 µM and 6 µM for THP-1) were selected based on the IC (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.) |
Databáze: | MEDLINE |
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