Development of a molecularly imprinted polymer-based electrochemical sensor for the selective detection of nerve agent VX metabolite ethyl methylphosphonic acid in human plasma and urine samples.

Autor: Sezigen S; Department of Medical CBRN Defense, University of Health Sciences, Ankara, Türkiye. sermet.sezigen@sbu.edu.tr., Kaya SI; Department of Analytical Chemistry, Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Türkiye., Bakirhan NK; Department of Analytical Chemistry, Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Türkiye., Ozkan SA; Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Türkiye.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Mar; Vol. 416 (6), pp. 1505-1515. Date of Electronic Publication: 2024 Jan 25.
DOI: 10.1007/s00216-024-05155-6
Abstrakt: This study focuses on the detection of ethyl methyl phosphonic acid (EMPA), a metabolite of the banned organophosphorus nerve agent VX. We developed an electrochemical sensor utilizing the molecularly imprinted polymer (MIP) based on 4-aminobenzoic acid (4-ABA) and tetraethyl orthosilicate for the selective detection of EMPA in human plasma and urine samples. The 4-ABA@EMPA/MIP/GCE sensor was constructed by a thermal polymerization process on a glassy carbon electrode and sensor characterization was performed by cyclic voltammetry and electrochemical impedance spectroscopy. The 4-ABA@EMPA/MIP/GCE sensor demonstrated impressive linear ranges 1.0 × 10 -10 M-2.5 × 10 -9 M for the standard solution, 1.0 × 10 -10 M-2.5 × 10 -9 M for the urine sample, and 1.0 × 10 -10 M-1 × 10 -9 M of EMPA for the plasma sample with outstanding detection limits of 2.75 × 10 -11 M (standard solution), 2.11 × 10 -11 M (urine), and 2.36 × 10 -11 M (plasma). The sensor exhibited excellent recovery percentages ranging from 99.86 to 101.30% in urine samples and 100.62 to 101.08% in plasma samples. These findings underscore the effectiveness of the 4-ABA@EMPA/MIP/GCE as a straightforward, highly sensitive, and selective interface capable of detecting the target analyte EMPA in human plasma and urine samples.
(© 2024. The Author(s).)
Databáze: MEDLINE
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