Development of injectable in situ hydrogels based on hyaluronic acid via Diels-Alder reaction for their antitumor activities studies.

Autor: Shi Y; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China. Electronic address: shiyongli2005@163.com., Xu S; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China., Zhao J; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China., Zhu H; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China., Pan X; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China., Zhao B; Basic Medicine College, Xinxiang Medical University, 453003, Xinxiang, PR China., Sun Z; First Clinical College, Xinxiang Medical University, 453003 Xinxiang, PR China., Li N; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China. Electronic address: jianjinshuln@163.com., Hou X; College of pharmacy, Xinxiang Medical University, 453003 Xinxiang, PR China; Pingyuan Laboratory, Xinxiang, Henan 453007, PR China. Electronic address: houxueyan1234@yeah.net.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Mar; Vol. 262 (Pt 1), pp. 129642. Date of Electronic Publication: 2024 Jan 23.
DOI: 10.1016/j.ijbiomac.2024.129642
Abstrakt: The objective of this study was to develop an injectable hydrogel based on furfuryl amine-conjugated hyaluronic acid (FA-conj-HA) and evaluate the in vivo anti-4 T1 tumor activity of doxorubicin-loaded hydrogel (DOX@FA-conj-HA gel ). The cargo-free hydrogel (FA-conj-HA gel ) was fabricated through a Diels-Alder reaction at 37 °C with FA-conj-HA as a gel material and four armed poly(ethylene glycol)2000-maleimide (4-arm-PEG2000-Mal) as a cross-linker. The bio-safety of FA-conj-HA gel were assessed, and the in vivo antitumor activity of DOX@FA-conj-HA gel was also investigated. Many 3D network structures were observed from scanning electron microscope (SEM) photograph, confirming the successful preparation of FA-conj-HA gel . The absence of cytotoxicity from FA-conj-HA gel was proved by the high viability of 4 T1 cells. In vivo bio-safety studies suggested that the obtained FA-conj-HA gel did not induce acute toxicity or other lesions in treated mice, confirming its high bio-safety. The reduced tumor volumes, hematoxylin-eosin staining (H&E), and TdT-mediated dUTP-biotin nick end labeling (TUNEL) analysis indicated the potent in vivo anti-4 T1 tumor effects of DOX@FA-conj-HA gel . In conclusion, the favorable bio-safety and potent antitumor activity of DOX@FA-conj-HA gel highlighted its potential application in oncological therapy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this study.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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