Transcriptomic profiles and diagnostic biomarkers in the Mediterranean seagrasses Posidonia oceanica and Cymodocea nodosa reveal mechanistic insights of adaptative strategies upon desalination brine stress.

Autor: Rodríguez-Rojas F; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile; Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha. Subida Leopoldo Carvallo 270, 2360004, Valparaíso, Chile. Valparaíso, Chile., Navarrete C; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile; Doctorado Interdisciplinario en Ciencias Ambientales, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha. Subida Leopoldo Carvallo 270, 2360004, Valparaíso, Chile., Rámila C; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile., Tapia-Reyes P; Escuela de Biotecnología, Facultad de Ciencias, Universidad Santo Tomás. Av. Ejército 146, 8370003, Santiago, Chile., Celis-Plá PSM; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile; Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha. Subida Leopoldo Carvallo 270, 2360004, Valparaíso, Chile. Valparaíso, Chile., González C; Escuela de Obras Civiles, Universidad Diego Portales. Av. Ejército 441, 8370191, Santiago, Chile., Pereira-Rojas J; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile; Doctorado Interdisciplinario en Ciencias Ambientales, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha. Subida Leopoldo Carvallo 270, 2360004, Valparaíso, Chile., Blanco-Murillo F; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile; Doctorado Interdisciplinario en Ciencias Ambientales, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha. Subida Leopoldo Carvallo 270, 2360004, Valparaíso, Chile; Departamento de Ciencias del Mar y Biología Aplicada, Universidad de Alicante, Carretera de San Vicente del Raspeig s/n, 03690, Alicante, Spain., Moreno P; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile., Gutiérrez-Campos C; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile., Sánchez-Lizaso JL; Departamento de Ciencias del Mar y Biología Aplicada, Universidad de Alicante, Carretera de San Vicente del Raspeig s/n, 03690, Alicante, Spain; Ciencias del Mar Universidad de Alicante, Unidad Asociada al CSIC por el IEO, Carretera de San Vicente del Raspeig s/n, 03690, Alicante, Spain., Sáez CA; Laboratorio de Investigación Ambiental Acuático, HUB AMBIENTAL UPLA, Universidad de Playa Ancha. Subida Leopoldo Carvallo 207, acceso Hospital del Salvador, 2360004, Valparaíso, Chile; Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha. Subida Leopoldo Carvallo 270, 2360004, Valparaíso, Chile. Valparaíso, Chile; Departamento de Ciencias del Mar y Biología Aplicada, Universidad de Alicante, Carretera de San Vicente del Raspeig s/n, 03690, Alicante, Spain. Electronic address: claudio.saez@ua.es.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Mar 15; Vol. 916, pp. 170326. Date of Electronic Publication: 2024 Jan 22.
DOI: 10.1016/j.scitotenv.2024.170326
Abstrakt: Seawater desalination by reverse osmosis is growing exponentially due to water scarcity. Byproducts of this process (e.g. brines), are generally discharged directly into the coastal ecosystem, causing detrimental effects, on benthic organisms. Understanding the cellular stress response of these organisms (biomarkers), could be crucial for establishing appropriate salinity thresholds for discharged brines. Early stress biomarkers can serve as valuable tools for monitoring the health status of brine-impacted organisms, enabling the prediction of long-term irreversible damage caused by the desalination industry. In this study, we conducted laboratory-controlled experiments to assess cellular and molecular biomarkers against brine exposure in two salinity-sensitive Mediterranean seagrasses: Posidonia oceanica and Cymodocea nodosa. Treatments involved exposure to 39, 41, and 43 psu, for 6 h and 7 days. Results indicated that photosynthetic performance remained unaffected across all treatments. However, under 43 psu, P. oceanica and C. nodosa exhibited lipid oxidative damage, which occurred earlier in P. oceanica. Additionally, P. oceanica displayed an antioxidant response at higher salinities by accumulating phenolic compounds within 6 h and ascorbate within 7 d; whereas for C. nodosa the predominant antioxidant mechanisms were phenolic compounds accumulation and total radical scavenging activity, which was evident after 7 d of brines exposure. Finally, transcriptomic analyses in P. oceanica exposed to 43 psu for 7 days revealed a poor up-regulation of genes associated with brassinosteroid response and abiotic stress response, while a high down-regulation of genes related to primary metabolism was detected. In C. nodosa, up-regulated genes were involved in DNA repair, cell cycle regulation, and reproduction, while down-regulated genes were mainly associated with photosynthesis and ribosome assembly. Overall, these findings suggest that 43 psu is a critical salinity-damage threshold for both seagrasses; and despite the moderate overexpression of several transcripts that could confer salt tolerance, genes involved in essential biological processes were severely downregulated.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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