Capillary electrophoresis tandem mass spectrometry to determine multiclass cyanotoxins in reservoir water and spinach samples.

Autor: Carmona-Molero R; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain., Aparicio-Muriana MM; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain., Lara FJ; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain., García-Campaña AM; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain., Olmo-Iruela MD; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain. Electronic address: mdolmo@ugr.es.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Feb 22; Vol. 1717, pp. 464666. Date of Electronic Publication: 2024 Jan 22.
DOI: 10.1016/j.chroma.2024.464666
Abstrakt: Cyanotoxins constitute a group of toxic secondary metabolites, the presence of which in any water body poses a major health risk. Moreover, advanced organisms such as edible plants exposed to these toxins, are a possible pathway for human exposure. Green analytical chemistry is demanding environmentally friendly analytical techniques. In this sense, we propose the use of capillary electrophoresis coupled to tandem mass spectrometry (CE-MS/MS) to determine a mixture of eight cyanotoxins belonging to three different classes: cyclic peptides (microcystin-LR, microcystin-RR and nodularin), alkaloids (cylindrospermopsin and anatoxin-a) and three isomeric non-protein amino acids (β-methylamino-l-alanine, 2,4-diaminobutyric acid and N-(2-aminoethyl)glycine). Separation was achieved by using an acidic background electrolyte consisting of 2 M formic acid and 20% acetonitrile in water. Parameters affecting MS/MS detection and the sheath-liquid interface were also studied. Finally, a combination of pH-junction, field-amplified sample stacking (FASS) and acid barrage as online preconcentration strategies, was employed to improve sensitivity and efficiency. The online preconcentration applied, in combination with a dual cartridge solid-phase extraction (SPE) system, allows to obtain limits of detection in the very low range of µg·L -1 for these multiclass cyanotoxins in reservoir water samples (from 0.005 to 0.10 µg·L -1 ). Furthermore, for the first time cyanotoxins are analysed in spinach samples through CE-MS/MS using the same SPE procedure, following lyophilisation and solid-liquid extraction with 6 mL 80 % aqueous MeOH.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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