Genomic epidemiology and molecular characteristics of bla NDM-1 -positive carbapenem-resistant Pseudomonas aeruginosa belonging to international high-risk clone ST773 in the Gauteng region, South Africa.

Autor: Jung H; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa., Pitout JDD; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.; Division of Microbiology, Alberta Public Laboratories, Cummings School of Medicine, University of Calgary, Calgary, Canada., Matsumura Y; Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan., Strydom KA; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.; Ampath National Reference Laboratory, Centurion, South Africa., Kingsburgh C; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.; Ampath National Reference Laboratory, Centurion, South Africa., Ehlers MM; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.; Department of Medical Microbiology, Tshwane Academic Division, National Health Laboratory Service (NHLS), Pretoria, South Africa., Kock MM; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa. marleen.kock@up.ac.za.; Department of Medical Microbiology, Tshwane Academic Division, National Health Laboratory Service (NHLS), Pretoria, South Africa. marleen.kock@up.ac.za.
Jazyk: angličtina
Zdroj: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2024 Apr; Vol. 43 (4), pp. 627-640. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1007/s10096-024-04763-5
Abstrakt: Purpose: The emergence of carbapenem-resistant P. aeruginosa (CRPA) harbouring acquired carbapenemase genes (bla VIM , bla IMP and bla NDM ) has become a global public health threat. Three CRPA isolates included in the study had an extensively drug-resistant phenotype with susceptibility to colistin only and were positive for the bla NDM-1 gene. The current study aimed to investigate the genomic epidemiology and molecular characteristics of the bla NDM-1 -positive CRPA isolates collected from the Gauteng region, South Africa.
Methods: Short read whole genome sequencing (WGS) was performed to determine sequence types (STs), genetic relatedness, resistome, virulome and the genetic environment of the bla NDM-1 gene.
Results: The WGS and phylogenetic analyses revealed that the study isolates belonged to an international high-risk clone ST773 and belonged to the same clade with eight bla NDM-1 -positive ST773 isolates from Hungary, India, Nigeria, South Korea and USA. The study isolates harboured a wide repertoire of intrinsic and acquired antibiotic resistance genes (ARGs) related with mobile genetic elements, porins and efflux pumps, as well as virulence factor genes. The clade-specific ARGs (bla NDM-1 , floR2/cmlA9, rmtB4, tetG) were found in a putative integrative and conjugative element (ICE) region similar to ICE6660-like.
Conclusion: As ICE carrying the bla NDM-1 gene can easily spread to other P. aeruginosa isolates and other Gram-negative bacteria, the findings in this study highlight the need for appropriate management strategies and active surveillance of CRPA isolates in the Gauteng region, South Africa.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: