| Autor: |
Zhou L; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Zhang Y; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Chen T; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Yun J; School of Life Sciences, Guangzhou University, Guangzhou 511370, Guangdong, China., Zhao M; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Zabed HM; School of Life Sciences, Guangzhou University, Guangzhou 511370, Guangdong, China., Zhang C; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Qi X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.; School of Life Sciences, Guangzhou University, Guangzhou 511370, Guangdong, China. |
| Abstrakt: |
Embracing the principles of sustainable development, the valorization of agrowastes into value-added chemicals has nowadays received significant attention worldwide. Herein, Escherichia coli was metabolically rewired to convert cellulosic hydrolysate of corn stover into a key platform chemical, namely, 3-hydroxypropionic acid (3-HP). First, the heterologous pathways were introduced into E. coli by coexpressing glycerol-3-P dehydrogenase and glycerol-3-P phosphatase in both single and fusion ( gpdp 12) forms, making the strain capable of synthesizing glycerol from glucose. Subsequently, a glycerol dehydratase (DhaB123-gdrAB) and an aldehyde dehydrogenase (GabD4) were overexpressed to convert glycerol into 3-HP. A fine-tuning between glycerol synthesis and its conversion into 3-HP was successfully established by 5'-untranslated region engineering of gpdp 12 and dhaB 123- gdrAB . The strain was further metabolically modulated to successfully prevent glycerol flux outside the cell and into the central metabolism. The finally remodulated chassis produced 32.91 g/L 3-HP from the cellulosic hydrolysate of stover during fed-batch fermentation. |
