Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration.

Autor:	Jackson CE; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom.; Insigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United Kingdom., Doyle I; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom., Khan H; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom., Williams SF; Department of Infection, Immunity and Cardiovascular Disease, Royal Hallamshire Hospital, The University of Sheffield, Sheffield, United Kingdom., Aldemir Dikici B; Department of Bioengineering, Izmir Institute of Technology, Urla, Türkiye., Barajas Ledesma E; Department of Chemistry, The University of Sheffield, Sheffield, United Kingdom., Bryant HE; School of Medicine and Population Health, University of Sheffield, Sheffield, United Kingdom., English WR; Norwich Medical School, University of East Anglia, Norwich, United Kingdom., Green NH; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom.; Insigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United Kingdom., Claeyssens F; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom.; Insigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Jan 08; Vol. 11, pp. 1321197. Date of Electronic Publication: 2024 Jan 08 (Print Publication: 2023).
DOI:	10.3389/fbioe.2023.1321197
Abstrakt:	Tumour survival and growth are reliant on angiogenesis, the formation of new blood vessels, to facilitate nutrient and waste exchange and, importantly, provide a route for metastasis from a primary to a secondary site. Whilst current models can ensure the transport and exchange of nutrients and waste via diffusion over distances greater than 200 μm, many lack sufficient vasculature capable of recapitulating the tumour microenvironment and, thus, metastasis. In this study, we utilise gelatin-containing polymerised high internal phase emulsion (polyHIPE) templated polycaprolactone-methacrylate (PCL-M) scaffolds to fabricate a composite material to support the 3D culture of MDA-MB-231 breast cancer cells and vascular ingrowth. Firstly, we investigated the effect of gelatin within the scaffolds on the mechanical and chemical properties using compression testing and FTIR spectroscopy, respectively. Initial in vitro assessment of cell metabolic activity and vascular endothelial growth factor expression demonstrated that gelatin-containing PCL-M polyHIPEs are capable of supporting 3D breast cancer cell growth. We then utilised the chick chorioallantoic membrane (CAM) assay to assess the angiogenic potential of cell-seeded gelatin-containing PCL-M polyHIPEs, and vascular ingrowth within cell-seeded, surfactant and gelatin-containing scaffolds was investigated via histological staining. Overall, our study proposes a promising composite material to fabricate a substrate to support the 3D culture of cancer cells and vascular ingrowth. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Jackson, Doyle, Khan, Williams, Aldemir Dikici, Barajas Ledesma, Bryant, English, Green and Claeyssens.)
Databáze:	MEDLINE
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