Genome binding properties of Zic transcription factors underlie their changing functions during neuronal maturation.

Autor:	Minto M; Duke University, Program in Computational Biology and Bioinformatics, Durham, NC 27710.; GenOmics and Translational Research Center, RTI International, Research Triangle Park, NC 27709., Sotelo-Fonseca JE; Duke University, Department of Neurobiology, Durham, NC 27710., Ramesh V; Duke University, Department of Neurobiology, Durham, NC 27710., West AE; Duke University, Department of Neurobiology, Durham, NC 27710.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jan 04. Date of Electronic Publication: 2024 Jan 04.
DOI:	10.1101/2024.01.04.574185
Abstrakt:	Background: The Zic family of transcription factors (TFs) promote both proliferation and maturation of cerebellar granule neurons (CGNs), raising the question of how a single, constitutively expressed TF family can support distinct developmental processes. Here we use an integrative experimental and bioinformatic approach to discover the regulatory relationship between Zic TF binding and changing programs of gene transcription during CGN differentiation. Results: We first established a bioinformatic pipeline to integrate Zic ChIP-seq data from the developing mouse cerebellum with other genomic datasets from the same tissue. In newborn CGNs, Zic TF binding predominates at active enhancers that are co-bound by developmentally-regulated TFs including Atoh1, whereas in mature CGNs, Zic TF binding consolidates toward promoters where it co-localizes with activity-regulated TFs. We then performed CUT&RUN-seq in differentiating CGNs to define both the time course of developmental shifts in Zic TF binding and their relationship to gene expression. Mapping Zic TF binding sites to genes using chromatin looping, we identified the set of Zic target genes that have altered expression in RNA-seq from Zic1 or Zic2 knockdown CGNs. Conclusion: Our data show that Zic TFs are required for both induction and repression of distinct, developmentally regulated target genes through a mechanism that is largely independent of changes in Zic TF binding. We suggest that the differential collaboration of Zic TFs with other TF families underlies the shift in their biological functions across CGN development. Competing Interests: Competing interests M.S.M – None J.E.S – None V.R. – None A.E.W - None
Databáze:	MEDLINE
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