Neu5Gc binding loss of subtype H7 influenza A virus facilitates adaptation to gallinaceous poultry following transmission from waterbirds but restricts spillback.

Autor: Guan M; Center for Influenza and Emerging Infectious Diseases (CIEID), University of Missouri, Columbia, MO, USA.; Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212.; Bond Life Sciences Center, University of Missouri, Columbia, MO, USA., Deliberto TJ; US Department of Agriculture Animal and Plant Health Inspection Service, Fort Collins, Colorado, USA., Feng A; Center for Influenza and Emerging Infectious Diseases (CIEID), University of Missouri, Columbia, MO, USA.; Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212.; Bond Life Sciences Center, University of Missouri, Columbia, MO, USA., Zhang J; Department of Bioengineering, Rice University, Houston, TX, 77030 USA., Li T; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Wang S; Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, Georgia, USA., Li L; Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, Georgia, USA., Killian ML; National Veterinary Services Laboratories, Veterinary Services, U.S. Department of Agriculture, Ames, Iowa, USA., Praena B; Center for Influenza and Emerging Infectious Diseases (CIEID), University of Missouri, Columbia, MO, USA.; Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212.; Bond Life Sciences Center, University of Missouri, Columbia, MO, USA., Giri E; Center for Influenza and Emerging Infectious Diseases (CIEID), University of Missouri, Columbia, MO, USA.; Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212.; Bond Life Sciences Center, University of Missouri, Columbia, MO, USA., Deliberto ST; US Department of Agriculture Animal and Plant Health Inspection Service, Fort Collins, Colorado, USA., Hang J; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Olivier A; Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA., Torchetti MK; National Veterinary Services Laboratories, Veterinary Services, U.S. Department of Agriculture, Ames, Iowa, USA., Tao YJ; Department of BioSciences, Rice University, Houston, TX 77251, USA., Parrish C; Department of Microbiology and Immunology, College of Veterinary Medicine, Baker Institute for Animal Health, Cornell University, Ithaca, NY, USA., Wan XF; Center for Influenza and Emerging Infectious Diseases (CIEID), University of Missouri, Columbia, MO, USA.; Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212.; Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.; Department of Electrical Engineering & Computer Science, College of Engineering, University of Missouri, Columbia, MO, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jan 03. Date of Electronic Publication: 2024 Jan 03.
DOI: 10.1101/2024.01.02.573990
Abstrakt: Migratory waterfowl, gulls, and shorebirds serve as natural reservoirs for influenza A viruses, with potential spillovers to domestic poultry and humans. The intricacies of interspecies adaptation among avian species, particularly from wild birds to domestic poultry, are not fully elucidated. In this study, we investigated the molecular mechanisms underlying avian species barriers in H7 transmission, particularly the factors responsible for the disproportionate distribution of poultry infected with A/Anhui/1/2013 (AH/13)-lineage H7N9 viruses. We hypothesized that the differential expression of N-glycolylneuraminic acid (Neu5Gc) among avian species exerts selective pressure on H7 viruses, shaping their evolution and enabling them to replicate and transmit efficiently among gallinaceous poultry, particularly chickens. Our glycan microarray and biolayer interferometry experiments showed that AH/13-lineage H7N9 viruses exclusively bind to Neu5Ac, in contrast to wild waterbird H7 viruses that bind both Neu5Ac and Neu5Gc. Significantly, reverting the V179 amino acid in AH/13-lineage back to the I179, predominantly found in wild waterbirds, expanded the binding affinity of AH/13-lineage H7 viruses from exclusively Neu5Ac to both Neu5Ac and Neu5Gc. When cultivating H7 viruses in cell lines with varied Neu5Gc levels, we observed that Neu5Gc expression impairs the replication of Neu5Ac-specific H7 viruses and facilitates adaptive mutations. Conversely, Neu5Gc deficiency triggers adaptive changes in H7 viruses capable of binding to both Neu5Ac and Neu5Gc. Additionally, we assessed Neu5Gc expression in the respiratory and gastrointestinal tissues of seven avian species, including chickens, Canada geese, and various dabbling ducks. Neu5Gc was absent in chicken and Canada goose, but its expression varied in the duck species. In summary, our findings reveal the crucial role of Neu5Gc in shaping the host range and interspecies transmission of H7 viruses. This understanding of virus-host interactions is crucial for developing strategies to manage and prevent influenza virus outbreaks in diverse avian populations.
Databáze: MEDLINE