Effects of photon irradiation in the presence and absence of hindlimb unloading on the behavioral performance and metabolic pathways in the plasma of Fischer rats.

Autor:	Raber J; Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States.; Departments of Neurology, and Radiation Medicine, Division of Neuroscience ONPRC, Oregon Health & Science University, Portland, OR, United States.; College of Pharmacy, Oregon State University, Corvallis, OR, United States., Holden S; Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States., Kessler K; Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States., Glaeser B; Neuroscience Center and Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, United States., McQuesten C; Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States., Chaudhari M; Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States., Stenzel F; Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States., Lenarczyk M; Radiation Biosciences Laboratory, Medical College of Wisconsin, Milwaukee, WI, United States., Leonard SW; Department of Radiation Oncology, Wake Forest School of Medicine, Winston-Salem, NC, United States., Morré J; Mass Spectrometry Core, Oregon State University, Corvallis, OR, United States., Choi J; Linus Pauling Institute, Oregon State University, Corvallis, OR, United States., Kronenberg A; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States., Borg A; Department of Radiation Oncology, Wake Forest School of Medicine, Winston-Salem, NC, United States., Kwok A; Department of Radiation Oncology, Wake Forest School of Medicine, Winston-Salem, NC, United States., Stevens JF; College of Pharmacy, Oregon State University, Corvallis, OR, United States.; Linus Pauling Institute, Oregon State University, Corvallis, OR, United States., Olsen C; Neuroscience Center and Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, United States., Willey JS; Department of Radiation Oncology, Wake Forest School of Medicine, Winston-Salem, NC, United States., Bobe G; Linus Pauling Institute, Oregon State University, Corvallis, OR, United States.; Department of Animal Sciences, Oregon State University, Corvallis, OR, United States., Minnier J; Oregon Health & Science University-Portland State University School of Public Health, Knight Cancer Institute Biostatistics Shared Resource, The Knight Cardiovascular Institute, OR Health & Science University, Portland, OR, United States., Baker JE; Neuroscience Center and Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2024 Jan 08; Vol. 14, pp. 1316186. Date of Electronic Publication: 2024 Jan 08 (Print Publication: 2023).
DOI:	10.3389/fphys.2023.1316186
Abstrakt:	Introduction: The space environment astronauts experience during space missions consists of multiple environmental challenges, including microgravity. In this study, we assessed the behavioral and cognitive performances of male Fisher rats 2 months after sham irradiation or total body irradiation with photons in the absence or presence of simulated microgravity. We analyzed the plasma collected 9 months after sham irradiation or total body irradiation for distinct alterations in metabolic pathways and to determine whether changes to metabolic measures were associated with specific behavioral and cognitive measures. Methods: A total of 344 male Fischer rats were irradiated with photons (6 MeV; 3, 8, or 10 Gy) in the absence or presence of simulated weightlessness achieved using hindlimb unloading (HU). To identify potential plasma biomarkers of photon radiation exposure or the HU condition for behavioral or cognitive performance, we performed regression analyses. Results: The behavioral effects of HU on activity levels in an open field, measures of anxiety in an elevated plus maze, and anhedonia in the M&M consumption test were more pronounced than those of photon irradiation. Phenylalanine, tyrosine, and tryptophan metabolism, and phenylalanine metabolism and biosynthesis showed very strong pathway changes, following photon irradiation and HU in animals irradiated with 3 Gy. Here, 29 out of 101 plasma metabolites were associated with 1 out of 13 behavioral measures. In the absence of HU, 22 metabolites were related to behavioral and cognitive measures. In HU animals that were sham-irradiated or irradiated with 8 Gy, one metabolite was related to behavioral and cognitive measures. In HU animals irradiated with 3 Gy, six metabolites were related to behavioral and cognitive measures. Discussion: These data suggest that it will be possible to develop stable plasma biomarkers of behavioral and cognitive performance, following environmental challenges like HU and radiation exposure. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Raber, Holden, Kessler, Glaeser, McQuesten, Chaudhari, Stenzel, Lenarczyk, Leonard, Morré, Choi, Kronenberg, Borg, Kwok, Stevens, Olsen, Willey, Bobe, Minnier and Baker.)
Databáze:	MEDLINE
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