Treponema pallidum protein Tp47 induced prostaglandin E2 to inhibit the phagocytosis in human macrophages.

Autor: Yi DY; Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China., Xu QY; Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China., He Y; Department of Medical Laboratory, The Second Affiliated Hospital of Xiamen Medical College, Xiamen, China., Zheng XQ; Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China., Yang TC; Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China., Lin Y; Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.
Jazyk: angličtina
Zdroj: Journal of the European Academy of Dermatology and Venereology : JEADV [J Eur Acad Dermatol Venereol] 2024 Jun; Vol. 38 (6), pp. 1166-1178. Date of Electronic Publication: 2024 Jan 23.
DOI: 10.1111/jdv.19809
Abstrakt: Background: During Treponema pallidum (T. pallidum) infection, the host's immune system actively engages in pursuit and elimination of T. pallidum, while T. pallidum skillfully employs various mechanisms to evade immune recognition. Macrophages exhibit incomplete clearance of T. pallidum in vitro and the underlying mechanism of how T. pallidum resists the attack of macrophage remains unclear.
Objectives: To investigate the effect of T. pallidum membrane protein Tp47 on the phagocytosis of macrophages.
Methods: THP-1-derived macrophages were used to investigate the role of Tp47 in the secretion of Prostaglandin E2 (PGE2) in macrophages and the mechanism by which Tp47 induced the production of PGE2, as well as the impact of PGE2 on the macrophage's phagocytosis.
Results: Tp47 (1-10 μg/mL) significantly inhibited the phagocytosis of latex beads and T. pallidum in macrophages (p ≤ 0.05). PGE2 production by macrophages could be induced by Tp47, and the phagocytic function of macrophages could be restored using PGE2 antibody. Tp47 produced PGE2 by activating the PERK/NF-κB/COX-2 pathway in macrophages. Inhibitors targeting PERK, NF-κB and COX-2, respectively, reduced the level of PGE2 and restored the phagocytic function of macrophages.
Conclusion: Tp47-induced PGE2 production via the PERK/NF-κB/COX-2 pathway contributed to macrophage phagocytosis inhibition, which potentially contributes to immune evasion during the T. pallidum infection.
(© 2024 European Academy of Dermatology and Venereology.)
Databáze: MEDLINE
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