Sequential Immunizations with Influenza Neuraminidase Protein Followed by Peptide Nanoclusters Induce Heterologous Protection.

Autor: Song WW; Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China., Wan MY; Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China., She JY; Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China., Zhao SL; Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China., Liu DJ; Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China., Chang HY; College of Life Sciences, Hunan Normal University, Changsha 410082, China., Deng L; Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China.; Beijing Weimiao Biotechnology Co., Ltd., Haidian District, Beijing 100093, China.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2024 Jan 03; Vol. 16 (1). Date of Electronic Publication: 2024 Jan 03.
DOI: 10.3390/v16010077
Abstrakt: Enhancing cross-protections against diverse influenza viruses is desired for influenza vaccinations. Neuraminidase (NA)-specific antibody responses have been found to independently correlate with a broader influenza protection spectrum. Here, we report a sequential immunization regimen that includes priming with NA protein followed by boosting with peptide nanoclusters, with which targeted enhancement of antibody responses in BALB/c mice to certain cross-protective B-cell epitopes of NA was achieved. The nanoclusters were fabricated via desolvation with absolute ethanol and were only composed of composite peptides. Unlike KLH conjugates, peptide nanoclusters would not induce influenza-unrelated immunity. We found that the incorporation of a hemagglutinin peptide of H2-d class II restriction into the composite peptides could be beneficial in enhancing the NA peptide-specific antibody response. Of note, boosters with N2 peptide nanoclusters induced stronger serum cross-reactivities to heterologous N2 and even heterosubtypic N7 and N9 than triple immunizations with the prototype recombinant tetrameric (rt) N2. The mouse challenge experiments with HK68 H3N2 also demonstrated the strong effectiveness of the peptide nanocluster boosters in conferring heterologous protection.
Databáze: MEDLINE
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