| Autor: |
Permyakova ES; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia., Solovieva AO; Research Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova St., Novosibirsk 630060, Russia., Sitnikova N; Research Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova St., Novosibirsk 630060, Russia., Kiryukhantsev-Korneev PV; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia., Kutzhanov MK; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia., Sheveyko AN; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia., Ignatov SG; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia.; State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Russia.; Lomonosov Moscow State University, GSP-1, 1 Leninskiye Gory, Moscow 119991, Russia., Slukin PV; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia.; State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Russia., Shtansky DV; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia., Manakhov AM; Research Laboratory 'Inorganic Nanomaterials', National University of Science and Technology 'MISIS', Moscow 119049, Russia.; Research Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova St., Novosibirsk 630060, Russia. |
| Abstrakt: |
Novel nanomaterials used for wound healing should have many beneficial properties, including high biological and antibacterial activity. Immobilization of proteins can stimulate cell migration and viability, and implanted Ag ions provide an antimicrobial effect. However, the ion implantation method, often used to introduce a bactericidal element into the surface, can lead to the degradation of vital proteins. To analyze the surface structure of nanofibers coated with a layer of plasma COOH polymer, fibronectin/gentamicin, and implanted with Ag ions, a new X-ray photoelectron spectroscopy (XPS) fitting method is used for the first time, allowing for a quantitative assessment of surface biomolecules. The results demonstrated noticeable changes in the composition of fibronectin- and gentamicin-modified nanofibers upon the introduction of Ag ions. Approximately 60% of the surface chemistry has changed, mainly due to an increase in hydrocarbon content and the introduction of up to 0.3 at.% Ag. Despite the significant degradation of fibronectin molecules, the biological activity of Ag-implanted nanofibers remained high, which is explained by the positive effect of Ag ions inducing the generation of reactive oxygen species. The PCL nanofibers with immobilized gentamicin and implanted silver ions exhibited very significant antipathogen activity to a wide range of Gram-positive and Gram-negative strains. Thus, the results of this work not only make a significant contribution to the development of new hybrid fiber materials for wound dressings but also demonstrate the capabilities of a new XPS fitting methodology for quantitative analysis of surface-related proteins and antibiotics. |
