| Autor: |
Francis JE; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Skakic I; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Majumdar D; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Taki AC; Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, VIC 3010, Australia., Shukla R; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Walduck A; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia., Smooker PM; School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia. |
| Abstrakt: |
In this study, novel solid lipid particles containing the adjuvant lipid monophosphoryl lipid A (termed 'SLN-A') were synthesised. The SLN-A particles were able to efficiently bind and form complexes with a DNA vaccine encoding the urease alpha subunit of Helicobacter pylori . The resultant nanoparticles were termed lipoplex-A. In a mouse model of H. pylori infection, the lipoplex-A nanoparticles were used to immunise mice, and the resultant immune responses were analysed. It was found that the lipoplex-A vaccine was able to induce high levels of antigen-specific antibodies and an influx of gastric CD4 + T cells in vaccinated mice. In particular, a prime with lipoplex-A and a boost with soluble UreA protein induced significantly high levels of the IgG1 antibody, whereas two doses of lipoplex-A induced high levels of the IgG2c antibody. In this study, lipoplex-A vaccination did not lead to a significant reduction in H. pylori colonisation in a challenge model; however, these results point to the utility of the system for delivering DNA vaccine-encoded antigens to induce immune responses and suggest the ability to tailor those responses. |
