Kirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids.

Autor:	Yang X; Department of Chemistry, Stanford University, Stanford, CA, USA.; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA., Forró C; Department of Chemistry, Stanford University, Stanford, CA, USA.; Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, Italy.; Institute for Biological I nformation Processing-Bioelectronics, IBI-3, Forschungszentrum Jülich, Jülich, Germany., Li TL; Department of Chemistry, Stanford University, Stanford, CA, USA.; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA., Miura Y; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA., Zaluska TJ; Department of Chemistry, Stanford University, Stanford, CA, USA., Tsai CT; Department of Chemistry, Stanford University, Stanford, CA, USA., Kanton S; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA., McQueen JP; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA., Chen X; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA., Mollo V; Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, Italy., Santoro F; Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, Italy.; Institute for Biological I nformation Processing-Bioelectronics, IBI-3, Forschungszentrum Jülich, Jülich, Germany.; Neuroelectronic Interfaces, RWTH Aachen, Aachen, Germany., Pașca SP; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA. spasca@stanford.edu.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA. spasca@stanford.edu., Cui B; Department of Chemistry, Stanford University, Stanford, CA, USA. bcui@stanford.edu.; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA. bcui@stanford.edu.
Jazyk:	angličtina
Zdroj:	Nature biotechnology [Nat Biotechnol] 2024 Dec; Vol. 42 (12), pp. 1836-1843. Date of Electronic Publication: 2024 Jan 22.
DOI:	10.1038/s41587-023-02081-3
Abstrakt:	Realizing the full potential of organoids and assembloids to model neural development and disease will require improved methods for long-term, minimally invasive recording of electrical activity. Current technologies, such as patch clamp, penetrating microelectrodes, planar electrode arrays and substrate-attached flexible electrodes, do not allow chronic recording of organoids in suspension, which is necessary to preserve architecture. Inspired by kirigami art, we developed flexible electronics that transition from a two-dimensional to a three-dimensional basket-like configuration with either spiral or honeycomb patterns to accommodate the long-term culture of organoids in suspension. Here we show that this platform, named kirigami electronics (KiriE), integrates with and enables chronic recording of cortical organoids for up to 120 days while preserving their morphology, cytoarchitecture and cell composition. We demonstrate integration of KiriE with optogenetic and pharmacological manipulation and modeling phenotypes related to a genetic disease. Moreover, KiriE can capture corticostriatal connectivity in assembloids following optogenetic stimulation. Thus, KiriE will enable investigation of disease and activity patterns underlying nervous system assembly. Competing Interests: Competing interests: Stanford University holds the patent for the generation of cortical organoids/spheroids (US11279914B2, listing S.P.P. as an inventor). Stanford University has filed a patent application that covers the devices, systems and methods of KiriE (S22-514 63/435,939, listing B.C., S.P.P., X.Y. and C.F. as inventors). The other authors declare no competing interests. (© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze:	MEDLINE
Externí odkaz:	Full text from SpringerLink