An improved pathway for autonomous bioluminescence imaging in eukaryotes.

Autor: Shakhova ES; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Karataeva TA; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Markina NM; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Mitiouchkina T; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Palkina KA; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Perfilov MM; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Wood MG; Promega Corporation, Madison, WI, USA., Hoang TT; Promega Corporation, Madison, WI, USA., Hall MP; Promega Corporation, Madison, WI, USA., Fakhranurova LI; Planta LLC, Moscow, Russia., Alekberova AE; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Malyshevskaia AK; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Gorbachev DA; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Bugaeva EN; Planta LLC, Moscow, Russia., Pletneva LK; Planta LLC, Moscow, Russia., Babenko VV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Boldyreva DI; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Gorokhovatsky AY; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Balakireva AV; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Gao F; Synthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK.; Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK., Choob VV; Planta LLC, Moscow, Russia.; Botanical Garden of Lomonosov Moscow State University, Moscow, Russia., Encell LP; Promega Corporation, Madison, WI, USA., Wood KV; Light Bio Inc, Ketchum, ID, USA., Yampolsky IV; Planta LLC, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.; Light Bio Inc, Ketchum, ID, USA.; Pirogov Russian National Research Medical University, Moscow, Russia., Sarkisyan KS; Planta LLC, Moscow, Russia. karen@light.bio.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. karen@light.bio.; Synthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK. karen@light.bio.; Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK. karen@light.bio.; Light Bio Inc, Ketchum, ID, USA. karen@light.bio., Mishin AS; Planta LLC, Moscow, Russia. alexander@planta.bio.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. alexander@planta.bio.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2024 Mar; Vol. 21 (3), pp. 406-410. Date of Electronic Publication: 2024 Jan 22.
DOI: 10.1038/s41592-023-02152-y
Abstrakt: The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: