Direct and indirect control of Rho-dependent transcription termination by the Escherichia coli lysC riboswitch.

Autor:	Ghosh T; Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, Quebec, Canada J1K 2R1., Jahangirnejad S; Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, Quebec, Canada J1K 2R1., Chauvier A; Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, Quebec, Canada J1K 2R1., Stringer AM; Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA., Korepanov AP; Expression Génétique Microbienne, UMR8261 CNRS, Université Paris Cité, Institut de Biologie Physico-Chimique, 75005 Paris, France., Côté JP; Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, Quebec, Canada J1K 2R1., Wade JT; Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA joseph.wade@health.ny.gov daniel.lafontaine@usherbrooke.ca.; Department of Biomedical Sciences, University at Albany, Albany, New York 12201, USA., Lafontaine DA; Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, Quebec, Canada J1K 2R1 joseph.wade@health.ny.gov daniel.lafontaine@usherbrooke.ca.
Jazyk:	angličtina
Zdroj:	RNA (New York, N.Y.) [RNA] 2024 Mar 18; Vol. 30 (4), pp. 381-391. Date of Electronic Publication: 2024 Mar 18.
DOI:	10.1261/rna.079779.123
Abstrakt:	Bacterial riboswitches are molecular structures that play a crucial role in controlling gene expression to maintain cellular balance. The Escherichia coli lysC riboswitch has been previously shown to regulate gene expression through translation initiation and mRNA decay. Recent research suggests that lysC gene expression is also influenced by Rho-dependent transcription termination. Through a series of in silico, in vitro, and in vivo experiments, we provide experimental evidence that the lysC riboswitch directly and indirectly modulates Rho transcription termination. Our study demonstrates that Rho-dependent transcription termination plays a significant role in the cotranscriptional regulation of lysC expression. Together with previous studies, our work suggests that lysC expression is governed by a lysine-sensing riboswitch that regulates translation initiation, transcription termination, and mRNA degradation. Notably, both Rho and RNase E target the same region of the RNA molecule, implying that RNase E may degrade Rho-terminated transcripts, providing a means to selectively eliminate these incomplete messenger RNAs. Overall, this study sheds light on the complex regulatory mechanisms used by bacterial riboswitches, emphasizing the role of transcription termination in the control of gene expression and mRNA stability. (© 2024 Ghosh et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
Databáze:	MEDLINE
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