Pathogen recognition pathway gene variants and inflammasome sensors gene expression in tuberculosis patients under treatment.

Autor: Borborema MEA; Laboratory of Human Genetics and Molecular Biology, Department of Genetics, Center for Biosciences, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, PE, 50670-901 - CEP, Brazil.; Keizo Asami Institute, Federal University of Pernambuco, Recife, PE, 50740-465 - CEP, Brazil., da Silva Santos AF; Laboratory of Human Genetics and Molecular Biology, Department of Genetics, Center for Biosciences, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, PE, 50670-901 - CEP, Brazil.; Keizo Asami Institute, Federal University of Pernambuco, Recife, PE, 50740-465 - CEP, Brazil., de Lucena TMC; Laboratory of Human Genetics and Molecular Biology, Department of Genetics, Center for Biosciences, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, PE, 50670-901 - CEP, Brazil.; Keizo Asami Institute, Federal University of Pernambuco, Recife, PE, 50740-465 - CEP, Brazil., Crovella S; Keizo Asami Institute, Federal University of Pernambuco, Recife, PE, 50740-465 - CEP, Brazil., da Silva Rabello MC; Aggeu Magalhães Research Institute (FIOCRUZ/IPqAM), Federal University of Pernambuco, Recife, PE, 50740-465 - CEP, Brazil., de Azevêdo Silva J; Laboratory of Human Genetics and Molecular Biology, Department of Genetics, Center for Biosciences, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, PE, 50670-901 - CEP, Brazil. jaqueline.azevedo@ufpe.br.; Keizo Asami Institute, Federal University of Pernambuco, Recife, PE, 50740-465 - CEP, Brazil. jaqueline.azevedo@ufpe.br.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Jan 22; Vol. 51 (1), pp. 161. Date of Electronic Publication: 2024 Jan 22.
DOI: 10.1007/s11033-023-09155-0
Abstrakt: Background: Several epidemiological studies have suggested that genetic variations in encoding pattern recognition receptors (PRRs) genes such as Toll Like Receptors (TLRs) and their signaling products, may influence the susceptibility, severity and outcome of tuberculosis (TB). After sensing a pathogen, the cell responds producing an inflammatory response, to restrain the pathogen's successful course of infection. Herein we assessed single nucleotide polymorphisms (SNP) and gene expression from pathogen recognition and inflammasome pathways in Brazilian TB patients.
Methods and Results: For genetic association analysis we included MYD88 and TLR4, PRRs sensing proteins. Allele distribution for MYD88 rs6853 (A > G) and TLR4 rs7873784 (C > G) presented conserved among the tested samples with statistically differential distribution in TB patients versus controls. However, when testing according to sample ethnicity (African or Caucasian-derived individuals) we identified that the rs6853 G/G genotype was associated with a lower susceptibility to TB in Caucasian population. Meanwhile, the rs7873784 G/G genotype was associated with a higher TB susceptibility in Afro-descendant ethnicity individuals. We also aimed to verify MYD88 and the inflammasome genes NLRP1 and NLRC4 expression in order to connect to active TB and/or clinical aspects.
Conclusions: We identified that inflammasome gene expression in TB patients under treatment display a similar pattern as in healthy controls, indicating that TB treatment impairs NLRP1 inflammasome activation.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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