Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius .

Autor:	Gambelli L; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Environment, Science and Economy, University of Exeter, Exeter, United Kingdom., McLaren M; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Conners R; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Sanders K; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Gaines MC; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Clark L; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Gold VAM; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Kattnig D; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Environment, Science and Economy, University of Exeter, Exeter, United Kingdom., Sikora M; Department of Theoretical Biophysics, Max Planck Institute for Biophysics, Frankfurt, Germany.; Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland., Hanus C; Institute of Psychiatry and Neurosciences of Paris, Inserm UMR1266 - Université Paris Cité, Paris, France.; GHU Psychiatrie et Neurosciences de Paris, Paris, France., Isupov MN; Henry Wellcome Building for Biocatalysis, Biosciences, Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom., Daum B; Living Systems Institute, University of Exeter, Exeter, United Kingdom.; Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Jan 22; Vol. 13. Date of Electronic Publication: 2024 Jan 22.
DOI:	10.7554/eLife.84617
Abstrakt:	Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of Sulfolobus acidocaldarius . The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly. Competing Interests: LG, MM, RC, KS, MG, LC, VG, DK, MS, CH, MI, BD No competing interests declared (© 2024, Gambelli et al.)
Databáze:	MEDLINE
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