| Autor: |
Arroyo G; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru.; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima 15202, Peru., Lescano AG; School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima 15202, Peru., Gavidia CM; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru., Lopez-Urbina T; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru., Ara-Gomez M; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru., Gomez-Puerta LA; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru., Bustos JA; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima 15202, Peru.; Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima 15030, Peru., Jayashi CM; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru., O'Neal SE; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima 15202, Peru.; School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, OR 97207, USA., Gonzalez AE; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru., Garcia HH; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima 15202, Peru.; Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima 15030, Peru., On Behalf Of The Cysticercosis Working Group In Peru Cwgp |
| Abstrakt: |
Enzyme-linked immunoelectrotransfer blot (EITB) detects antibodies against seven Taenia solium larvae antigens in three protein families (GP50, T24/42, and 8-kDa) with different structures and functions. EITB banding patterns against these antigens in pigs provide information about the course of infection and may discriminate viable cysticercosis. We analyzed the banding patterns and infection outcomes (presence of viable cysts, degenerated cysts, and any cysts) of 512 rural pigs. Banding patterns were grouped into homogenous classes using latent class analysis, and relationships with infection outcomes were assessed. Four classes were identified: 1 ( n = 308, EITB-negative or positive for the GP50 family), 2 ( n = 127, positive for GP50 (GP50 family), GP42-39 and GP24 (T24/42 family), but negative for 8-kDa antigens), 3 ( n = 22, positive for GP50 and T24/42 antigens (GP42-39 and GP24), as well as to 8-kDa bands GP13, GP14, and GP18, but negative for GP21), and 4 ( n = 55, positive for GP50 and T24/42 antigens, as well as to 8-kDa antigens GP21 and GP18 in combination). Pigs in classes 3 and 4 were more likely to have viable cysts (72.6% and 96.4%, respectively) than pigs in classes 1 and 2 (0.7% and 27.6%, respectively; p < 0.001). The number of infections with any cysts was higher in classes 3 and 4 (77.3% and 98.2%, respectively) and lower in classes 2 and 1 (34.7% and 4.9%, respectively; p < 0.001). Pigs with viable cysts represented >90% of pigs with any cysts in classes 3 and 4 (94.1% and 98.2%, respectively), while degenerated cysts were frequent among pigs with any cysts in classes 1, 3, and 2 (86.7%, 47.1%, and 43.2%, respectively; p < 0.001). EITB banding patterns strongly correlate with cysticercosis infection status in rural pigs, with classes 3 and 4 being more predictive of viable infections. |
