| Autor: |
Suwankitwat N; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Deemagarn T; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Bhakha K; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Songkasupa T; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Vitoonpong R; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Trakunjaroonkit P; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Rodphol S; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Nuansrichay B; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Chintapitaksakul L; National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand., Wongsarattanasin K; Animal Health Development Group, Lopburi Provincial Livestock Office, Department of Livestock Development, Lopburi 15000, Thailand., Kwon OK; Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea., Kang HE; Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea., Shin YK; Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. |
| Abstrakt: |
Lumpy skin disease (LSD) is a viral infection that impacts the cattle industry. The most efficient approach to prevent disease involves the utilization of live-attenuated LSD vaccines (LAVs), which stands out as the most successful method. However, LAVs might be subjected to changes to their genomes during replication that increase viral infectivity or virulence. The objective of this study was to monitor alterations in the genetic characteristics of the lumpy skin disease virus (LSDV) in beef cattle following the administration of LAVs in Lopburi Province of Central Thailand. A total of four skin samples from LSD cases were collected from non-vaccinated animals that exhibited LSD clinical symptoms from two distinct districts, spanning three subdistricts within the region. The samples of cattle were analyzed using real-time PCR targeting the LSDV074 p32 gene, the virus was isolated, and the entire genome sequences were evaluated through a single nucleotide polymorphisms (SNPs) analysis, and phylogenetic trees were assembled. The investigations revealed that LSDVs from two isolates from Chai Badan district exhibited significant mutations in the open reading frame (ORF) 023 putative protein, while another two isolates from Lam Sonthi district had a change in the untranslated region (UTR). For a result, the most proficient disease diagnosis and control should be evaluated on viral genetics on a regular basis. |
