Differences in the detection of circulating Hsp90 alpha between patients with atopic dermatitis and dermatitis herpetiformis.
| Autor: | Sitko K; Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, Poland., Kárpáti S; Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, Budapest, Hungary., Węgrzyn G; Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, Poland., Mincewicz G; Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, Poland., Trzeciak M; Department of Dermatology, Venereology and Allergology, Medical University of Gdańsk, Gdańsk, Poland., Kasperkiewicz M; Department of Dermatology, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States., Tukaj S; Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, Poland. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in medicine [Front Med (Lausanne)] 2024 Jan 05; Vol. 10, pp. 1327144. Date of Electronic Publication: 2024 Jan 05 (Print Publication: 2023). |
| DOI: | 10.3389/fmed.2023.1327144 |
| Abstrakt: | Heat shock protein 90 alpha (Hsp90α) is one of the key intra- and extracellular chaperones responsible for the biological activity of various signaling molecules that are involved in (auto)immune-mediated inflammatory diseases. Recent epidemiologic data suggest that patients with atopic dermatitis (AD) are at risk for several autoimmune diseases, including dermatitis herpetiformis (DH), an extraintestinal manifestation of celiac disease (CD). In addition, pruritic diseases such as AD may be confused clinically with DH. In this study, we aimed to determine the role of circulating Hsp90α in patients with AD in relation to patients with DH, CD, and healthy controls. Using an enzyme-linked immunosorbent assay, levels of circulating Hsp90α were determined in serum samples derived from patients with AD ( n = 31), DH ( n = 26), CD ( n = 15), and healthy controls ( n = 55). Although serum concentrations of Hsp90α were similar between patients with DH, CD, and healthy controls, we found that serum levels of Hsp90α were significantly higher (mean value of 5.08-fold; p < 0.0001) in patients with AD when compared to patients with DH. A cutoff value calculated as 2 × standard deviation above the mean concentration of Hsp90α in DH patients revealed that 83.9% (26/31) of AD patients were Hsp90α positive, whereas none of the DH patients (0/26) displayed such a positivity. This preliminary study suggests a distinct role for extracellular Hsp90α in the pathogenesis of AD compared to DH and its potential use in distinguishing AD from DH. Nevertheless, the potential role of the evaluation of extracellular Hsp90α for distinguishing between AD and DH is at present speculative and requires further and careful observations. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Sitko, Kárpáti, Węgrzyn, Mincewicz, Trzeciak, Kasperkiewicz and Tukaj.) |
| Databáze: | MEDLINE |
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