| Autor: |
Zhdanov G; Chemistry Department of Lomonosov Moscow State University, 119991 Moscow, Russia.; Moscow Institute of Physics and Technology, Institute of Quantum Technologies, 141700 Dolgoprudny, Russia., Gambaryan A; Chumakov Federal Scientific Centre for Research and Development of Immune and Biological Products RAS, 108819 Moscow, Russia., Akhmetova A; Physics Department of Lomonosov Moscow State University, 119991 Moscow, Russia., Yaminsky I; Physics Department of Lomonosov Moscow State University, 119991 Moscow, Russia., Kukushkin V; Osipyan Institute of Solid State Physics of the Russian Academy of Science, 142432 Chernogolovka, Russia., Zavyalova E; Chemistry Department of Lomonosov Moscow State University, 119991 Moscow, Russia.; Moscow Institute of Physics and Technology, Institute of Quantum Technologies, 141700 Dolgoprudny, Russia. |
| Abstrakt: |
Surface-enhanced Raman spectroscopy (SERS)-based aptasensors for virus determination have attracted a lot of interest recently. This approach provides both specificity due to an aptamer component and a low limit of detection due to signal enhancement by a SERS substrate. The most successful SERS-based aptasensors have a limit of detection (LoD) of 10-100 viral particles per mL (VP/mL) that is advantageous compared to polymerase chain reactions. These characteristics of the sensors require the use of complex substrates. Previously, we described silver nanoisland SERS-substrate with a reproducible and uniform surface, demonstrating high potency for industrial production and a suboptimal LoD of 4 × 10 5 VP/mL of influenza A virus. Here we describe a study of the sensor morphology, revealing an unexpected mechanism of signal enhancement through the distortion of the nanoisland layer. A novel modification of the aptasensor was proposed with chromium-enhanced adhesion of silver nanoparticles to the surface as well as elimination of the buffer-dependent distortion-triggering steps. As a result, the LoD of the Influenza A virus was decreased to 190 VP/mL, placing the nanoisland SERS-based aptasensors in the rank of the most powerful sensors for viral detection. |
