Anti- Salmonella Defence and Intestinal Homeostatic Maintenance In Vitro of a Consortium Containing Limosilactobacillus fermentum 3872 and Ligilactobacillus salivariu s 7247 Strains in Human, Porcine, and Chicken Enterocytes.

Autor: Abramov VM; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia.; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Kosarev IV; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia.; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Machulin AV; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Deryusheva EI; Institute for Biological Instrumentation, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Priputnevich TV; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Panin AN; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Chikileva IO; Blokhin National Research Center of Oncology, Ministry of Health RF, 115478 Moscow, Russia., Abashina TN; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Manoyan AM; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Akhmetzyanova AA; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Blumenkrants DA; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Ivanova OE; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Papazyan TT; Alltech Company Moscow, 105062 Moscow, Russia., Nikonov IN; Federal State Educational Institution of Higher Professional Education, Moscow State Academy of Veterinary Medicine and Biotechnology Named after K.I. Skryabin, 109472 Moscow, Russia., Suzina NE; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Melnikov VG; Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia., Khlebnikov VS; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Sakulin VK; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Samoilenko VA; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Gordeev AB; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Sukhikh GT; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Uversky VN; Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA., Karlyshev AV; Department of Biomolecular Sciences, School of Life Sciences, Chemistry and Pharmacy, Faculty of Health, Science, Social Care and Education, Kingston University London, Kingston upon Thames KT1 2EE, UK.
Jazyk: angličtina
Zdroj: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2023 Dec 28; Vol. 13 (1). Date of Electronic Publication: 2023 Dec 28.
DOI: 10.3390/antibiotics13010030
Abstrakt: Limosilactobacillus fermentum strain 3872 (LF3872) was originally isolated from the breast milk of a healthy woman during lactation and the breastfeeding of a child. Ligilactobacillus salivarius strain 7247 (LS7247) was isolated at the same time from the intestines and reproductive system of a healthy woman. The genomes of these strains contain genes responsible for the production of peptidoglycan-degrading enzymes and factors that increase the permeability of the outer membrane of Gram-negative pathogens. In this work, the anti- Salmonella and intestinal homeostatic features of the LF3872 and LS7247 consortium were studied. A multi-drug resistant (MDR) strain of Salmonella enteritidis (SE) was used in the experiments. The consortium effectively inhibited the adhesion of SE to intact and activated human, porcine, and chicken enterocytes and reduced invasion. The consortium had a bactericidal effect on SE in 6 h of co-culturing. A gene expression analysis of SE showed that the cell-free supernatant (CFS) of the consortium inhibited the expression of virulence genes critical for the colonization of human and animal enterocytes. The CFS stimulated the production of an intestinal homeostatic factor-intestinal alkaline phosphatase (IAP)-in Caco-2 and HT-29 enterocytes. The consortium decreased the production of pro-inflammatory cytokines IL-8, TNF-α, and IL-1β, and TLR4 mRNA expression in human and animal enterocytes. It stimulated the expression of TLR9 in human and porcine enterocytes and stimulated the expression of TLR21 in chicken enterocytes. The consortium also protected the intestinal barrier functions through the increase of transepithelial electrical resistance (TEER) and the inhibition of paracellular permeability in the monolayers of human and animal enterocytes. The results obtained suggest that a LF3872 and LS7247 consortium can be used as an innovative feed additive to reduce the spread of MDR SE among the population and farm animals.
Databáze: MEDLINE
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