An endoscopic approach providing near-infrared laser-induced coagulation with accurate depth limits.

Autor: Turker-Burhan M; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Türkiye., Ellidokuz EB; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Department of Internal Medicine Gastroenterology, Faculty of Medicine, Dokuz Eylul University, Izmir, Türkiye., Bagriyanik HA; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Department of Histology and Embryology, Faculty of Medicine, Dokuz Eylul University, Izmir, Türkiye., Tozburun S; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Türkiye.; Department of Biophysics, Faculty of Medicine, Dokuz Eylul University, Izmir, Türkiye.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2024 Apr; Vol. 17 (4), pp. e202300377. Date of Electronic Publication: 2024 Jan 21.
DOI: 10.1002/jbio.202300377
Abstrakt: This article investigates an endoscopic approach that utilizes negative pressure to achieve laser-induced thermal coagulation limited to the esophageal wall's mucosal and superficial submucosal layers. The study was built upon a series of studies combining numerical simulation based on the Monte-Carlo technique and ex vivo porcine tissue experiments, including apparatus design and histology analysis. An endoscopy apparatus was developed using 3D printing to validate the tissue stretching-based approach. A fiber-pigtailed diode was used as the near-infrared source, emitting 208.8 W/cm 2 laser irradiance at 1.5 μm. Simulation results suggested that the approach successfully created a local heat well to prevent residual thermal effects (>65°C) from penetrating the deeper submucosal layer. Histology analysis of ex vivo tissues showed that at a fluence of 5.22 kJ/cm 2 , the depth of thermal coagulation was reduced by half compared to the control. With further preclinical studies, including endoscopy apparatus design, the approach can be applied to the larger esophageal surface.
(© 2024 The Authors. Journal of Biophotonics published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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