Inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (Oryza sativa).

Autor: Chowdhury AR; ICAR-National Institute of Secondary Agriculture, Namkum, Ranchi, 834 010, Jharkhand, India., Kumar R; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Mahanty A; ICAR-National Rice Research Institute, Cuttack, Odisha, 753006, India., Mukherjee K; Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India., Kumar S; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Tribhuvan KU; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Sheel R; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India.; Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India., Lenka S; ICAR-National Rice Research Institute, Cuttack, Odisha, 753006, India., Singh BK; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Chattopadhyay C; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Sharma TR; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Bhadana VP; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India., Sarkar B; ICAR-Indian Institute of Agricultural Biotechnology, GarhkhatangaRanchi, Jharkhand, 834 003, India. biplab_puru@yahoo.co.in.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jan 20; Vol. 14 (1), pp. 1779. Date of Electronic Publication: 2024 Jan 20.
DOI: 10.1038/s41598-023-49918-0
Abstrakt: Rice (Oryza sativa) being among the most important food crops in the world is also susceptible to various bacterial and fungal diseases that are the major stumbling blocks in the way of increased production and productivity. The bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae and the sheath blight disease caused by Rhizoctonia solani are among the most devastating diseases of the rice crop. In spite of the availability of array of chemical control, there are chances of development of resistance. Thus, there is a need for the nanotechnological intervention for management of disease in the form of copper and silver nano-composites. The copper (CuNPs) and silver nanoparticles (AgNPs) were synthesized using green route and characterized using different high throughput techniques, i.e., UV-Vis, FT-IR, DLS, XRD, FE-SEM, TEM. The particle size and zeta potential of synthesized CuNPs and AgNPs were found 273 nm and - 24.2 mV; 95.19 nm and - 25.5 mV respectively. The nanocomposite of CuNPs and AgNPs were prepared having particle size in the range of 375-306 nm with improved stability (zeta potential - 54.7 to - 39.4 mV). The copper and silver nanoparticle composites evaluated against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani were found to have higher antibacterial (inhibition zone 13 mm) and antifungal activities (77%) compared to only the copper nanoparticle (8 mm; 62% respectively). Net house trials of nano-composite formulations against the bacterial blight of rice also corroborated the potential of nanocomposite formulation. In silico studies were carried out selecting two disease-causing proteins, peptide deformylase (Xanthomonas oryzae) and pectate lyase (Rhizoctonia solani) to perform the molecular docking. Interaction studies indicatedthat both of these proteins generated better complex with CuNPs than AgNPs. The study suggested that the copper and silver nano-composites could be used for developing formulations to control these devastating rice diseases.
(© 2023. The Author(s).)
Databáze: MEDLINE
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