Metabolic Profiling, GC-MS, LC-ESI-MS/MS Analysis, Phenolics Isolation and Biological Evaluation of the Aerial Parts Extracts of Felicia abyssinica L.

Autor: Elshorbagy AM; Department of Pharmacognosy, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897, Egypt., Fayed MAA; Department of Pharmacognosy, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897, Egypt., Sallam A; Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt., Badria FA; Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Mar; Vol. 21 (3), pp. e202301347. Date of Electronic Publication: 2024 Feb 15.
DOI: 10.1002/cbdv.202301347
Abstrakt: Felicia abyssinica L., family Asteraceae, is widely used in folk medicine. This represents the first study to investigate its phytoconstituents and pharmacological effects. Phytoconstituents identified by GC-MS, LC-ESI-MS/MS-based metabolomics, and NMR (1D & 2D). GC-MS of the (FAMEs) revealed mainly the identification of 55 fatty acids. LC-ESI-MS/MS analysis resulted in the tentative identity of 13 compounds representing flavonoids, phenolics, and fatty acids. Ethyl acetate fraction exhibited the highest total flavonoids 66.19 mg/mL Rutin equivalent, while the methanolic fraction showed the highest phenolics 87.70 mg/mL gallic acid equivalent, and the total condensed tannins were 64.35 μg CE/mg catechins equivalent. A flavonoid and a cinnamic acid derivative were identified as quercetin 3-O-(2'''-O-acetyl) rutinoside (Mumikotin A) (1) and Methyl sinapate (2). Biological evaluation of antioxidant and cytotoxic activities was carried out. Cytotoxicity was examined on HepG-2 cell lines where the average cell viability was 91.42 % and 52.48 % for concentrations 10 and 100 μg/mL respectively. Methylene chloride and methanolic fractions showed the highest antioxidant activity 225 μg/mL Ascorbic acid equivalents. It is hypothesized that high phenolics, flavonoid content, and oxygenated identified compounds contribute to the antioxidant activity and can be regarded as a promising species for nutraceuticals active antioxidants with potential value for remedy.
(© 2024 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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